J Korean Med Sci.
2004 Jun;19(3):333-340. 10.3346/jkms.2004.19.3.333.
Bacterial Growth in Amniotic Fluid Is Dependent on the Iron-Availability and the Activity of Bacterial Iron-Uptake System
- Affiliations
-
- 1Department of Pediatrics, Seonam University Medical School, Namwon, Korea.
- 2Department of Pediatrics, Chosun University Medical School, Gwangju, Korea.
- 3Department of Anatomy, Chosun University Medical School, Gwangju, Korea.
- 4Department of Microbiology and Research Center for Resistant Cells, Chosun University Medical School, Gwangju, Korea. shsin@chosun.ac.kr
- KMID: 1786808
- DOI: http://doi.org/10.3346/jkms.2004.19.3.333
Abstract
- In the present study, the relationship among iron-availability, antibacterial activity, role of meconium as an iron source and the activity of bacterial iron-uptake system (IUS) for bacterial growth in amniotic fluid (AF) were investigated. Staphylococcus aureus ATCC 6538 and its streptonigrin-resistant (SR) mutant with defective IUS were used as the test strains. The growth of S. aureus in AF was stimulated dosedependently by addition of meconium. Bacterial growth stimulated by meconium was re-inhibited dose-dependently by addition of iron-chelator, dipyridyl and apotransferrin. Iron concentration was correlated with the meconium content in AF (r(2)= 0.989, p=0.001). High-affinity IUS of S. aureus was expressed only in AF but not in AF with meconium. The growth of SR strain was more retarded than that of the parental strain in the iron-deficient brain heart infusion (ID-BHI), clear AF and AF containing apotransferrin. The retarded growth of both strains in the ID-BHI and AF was recovered by addition of holotransferrin, hemoglobin and FeCl3. Taken together, the antibacterial activity of AF is closely related with low iron-availability. Bacterial growth in AF considerably depends on the activity of bacterial IUS. Meconium acts as one of the exogenous iron-sources and thus can stimulate bacterial growth in AF.
MeSH Terms
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Amniotic Fluid/*microbiology
Antibiotics, Antineoplastic/pharmacology
Chelating Agents/pharmacology
Dose-Response Relationship, Drug
Female
Ferric Compounds/pharmacology
Human
Iron/*metabolism
Ligands
Meconium/metabolism
Mutation
Pregnancy
Pregnancy Trimester, Third
Protein Binding
Staphylococcus aureus/metabolism
Streptonigrin/pharmacology
Support, Non-U.S. Gov't
Time Factors
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