J Korean Med Sci.  2004 Feb;19(1):69-73. 10.3346/jkms.2004.19.1.69.

Airway Inflammation and Allergen Specific IgE Production May Persist Longer Than Airway Hyperresponsiveness in Mice

• Chang YS
• Kim YK
• Kim TB
• Kang HR
• Kim SS
• Bahn JW
• Min KU
• Kim YY
• Cho SH

• KMID: 1785696
• DOI: http://doi.org/10.3346/jkms.2004.19.1.69

Abstract

During the preclinical study of new therapeutic modality, we evaluate whether the treatment can reverse the established asthma phenotypes in animal model. However, few have reported on the long term persistence of asthma phenotypes upon re-challenge with allergen (secondary challenge) in animal model. We evaluated the persistence of asthma phenotypes by secondary challenge at different times in previously challenged murine asthma model. BALB/c mice sensitized by intraperitoneal injections of 20 microgram of ovalbumin and 1 mg of alum on days 1 and 14 were challenged initially by the inhalation of 1% ovalbumin for 30 min on days 21, 22, and 23. Each group of mice was rechallenged at 5, 7, 9, or 12 weeks after the initial challenge. Airway hyperresponsiveness, BAL fluid, airway histology and serum ovalbumin-specific IgE level were evaluated. Airway eosinophilia, airway inflammation and serum ovalbumin-specific IgE production persisted upon secondary allergen challenges at least 12 weeks after the initial challenge. However, airway hyperresponsiveness persisted only until mice were rechallenged 7 weeks after the initial challenge. Airway inflammation and allergen specific IgE production may persist longer than airway hyperresponsiveness in a mouse asthma model of secondary allergen challenge.

Keyword

Asthma; Disease Models; Animal; Mice; Allergen Challenge

MeSH Terms

Allergens
Animals
Asthma/metabolism/*pathology
Bronchial Hyperreactivity/*diagnosis
Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid
Female
Immunoglobulin E/*biosynthesis/chemistry
*Inflammation
Lung/pathology
Mice
Mice, Inbred BALB C
Ovalbumin/pharmacology
Phenotype
Respiratory Hypersensitivity/*diagnosis
Respiratory System/pathology
Support, Non-U.S. Gov't
Time Factors

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