J Periodontal Implant Sci.  2012 Aug;42(4):113-118. 10.5051/jpis.2012.42.4.113.

The biological effects of fibrin-binding synthetic oligopeptides derived from fibronectin on osteoblast-like cells

• Kim YJ
• Park YJ
• Lee YM
• Rhyu IC
• Ku Y

• KMID: 1783646
• DOI: http://doi.org/10.5051/jpis.2012.42.4.113

Abstract

PURPOSE
The aim of this study was to investigate the effects of synthetic fibronectin (FN) fragments, including fibrin binding sites from amino-terminal FN fragments containing type I repeats 1 to 5, on osteoblast-like cell activity.
METHODS
Oligopeptides ranging from 9 to 20 amino acids, designated FF1, FF3, and FF5, were synthesized by a solid-phase peptide synthesizing system, and we investigated the effects of these peptides on cell attachment and extent of mineralization using confocal microscopy, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, and Alizarin red S staining.
RESULTS
FF3 and FF5 peptides increased the number of attached human osteoblastic cells, and FF3 administration led to prominent cell spreading. Mineralization was increased in FF3 and FF5 compared to FF1 and the untreated control.
CONCLUSIONS
Taken together, it can be concluded that the fibrin-binding oligopeptides FF3 and FF5 enhanced cell attachment and mineralization on osteoblast-like cells. These results indicate that FF3 and FF5 have the potential to increase osteoblast-like cell activity. Performing an in vivo study may provide further possibilities for surface modification of biomimetic peptides to enhance osteogenesis, thus improving the regeneration of destroyed alveolar bone.

Keyword

Fibrin; Fibronectins; Oligopeptides; Osteoblasts

MeSH Terms

Amino Acids
Anthraquinones
Binding Sites
Biomimetics
Fibrin
Fibronectins
Humans
Microscopy, Confocal
Oligopeptides
Osteoblasts
Osteogenesis
Peptides
Regeneration
Tetrazolium Salts
Thiazoles
Amino Acids
Anthraquinones
Fibrin
Fibronectins
Oligopeptides
Peptides
Tetrazolium Salts
Thiazoles

Figure

• 

  Figure 1 Humanosteoblastic cell attachment using confocal laser scanning microscopy. Nuclei are blue, and actin cytoskeletons are red. (A) Not treated (NT), (B) FF1, (C) FF3, (D) FF5.

• 

  Figure 2 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay of humanosteoblastic cell attachment after 12 hours. NT: not treated.

• 

  Figure 3 Humanosteoblastic cell mineralization to FF1, 3, and 5 measured by confocal laser scanning microscopy. Each micrograph shows mineralization by calcein (green), nuclei (blue), actin filaments (red). (A) Not treated (NT), (B) FF1, (C) FF3, (D) FF5.

• 

  Figure 4 Alizarin red S stained wells of humanosteoblastic cells cultured for 10 days in peptides FF1, 3, and 5. (A) Not treated NT, (B) FF1, (C) FF3, (D) FF5 (×20).

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