J Korean Med Sci.
2009 Oct;24(5):853-859. 10.3346/jkms.2009.24.5.853.
Differences in Circulating Dendritic Cell Subtypes in Pregnant Women, Cord Blood and Healthy Adult Women
- Affiliations
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- 1Department of Laboratory Medicine, Seoul National University Boramae Hospital, Seoul, Korea.
- 2Seoul Metropolitan Public Cord Blood Bank, Seoul, Korea.
- 3Department of Family Medicine, Seoul National University Boramae Hospital, Seoul, Korea.
- 4Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
- 5Department of Laboratory Medicine, College of Medicine, Konkuk University, Seoul, Korea. eysongmd@hanmail.net
- 6Department of Biomedical Science and Technology, College of Medicine, Konkuk University, Seoul, Korea.
- 7Department of Respiratory Medicine, College of Medicine, Konkuk University, Seoul, Korea.
- KMID: 1782006
- DOI: http://doi.org/10.3346/jkms.2009.24.5.853
Abstract
- Different subtypes of dendritic cells (DC) influence the differentiation of naive T lymphocytes into T helper type 1 (Th1) and Th2 effector cells. We evaluated the percentages of DC subtypes in peripheral blood from pregnant women (maternal blood) and their cord blood compared to the peripheral blood of healthy non pregnant women (control). Circulating DC were identified by flow cytometry as lineage (CD3, CD14, CD16, CD19, CD20, and CD56)-negative and HLA-DR-positive cells. Subtypes of DC were further characterized as myeloid DC (CD11c+/CD123+/-), lymphoid DC (CD11c-/CD123+++) and less differentiated DC (CD11c-/CD123+/-). The frequency of DC out of all nucleated cells was significantly lower in maternal blood than in control (P<0.001). The ratio of myeloid DC/lymphoid DC was significantly higher in maternal blood than in control (P<0.01). HLA-DR expressions of myeloid DC as mean fluorescence intensity (MFI) were significantly less in maternal blood and in cord blood than in control (P<0.001, respectively). The DC differentiation factors, TNF-alpha and GM-CSF, released from mononuclear cells after lipopolysaccharide stimulation were significantly lower in maternal blood than in control (P<0.01). The distribution of DC subtypes was different in maternal and cord blood from those of non-pregnant women. Their role during pregnancy remains to be determined.
Keyword
MeSH Terms
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Adult
Cell Differentiation
Dendritic Cells/*classification/cytology/immunology
Female
Fetal Blood/cytology/*immunology
Flow Cytometry
Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
HLA-DR Antigens/metabolism
Humans
Lipopolysaccharides/pharmacology
Lymphocyte Activation
Pregnancy
T-Lymphocytes/cytology/immunology
Th1 Cells/cytology/immunology
Th2 Cells/cytology/immunology
Tumor Necrosis Factor-alpha/metabolism
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