Yonsei Med J.  1983 Jun;24(1):1-5. 10.3349/ymj.1983.24.1.1.

Detection of Cancer by Culturing Cancerous Tissue In Plastico

• Lee WY
• Kim JD
• Kim BS

• KMID: 1758482
• DOI: http://doi.org/10.3349/ymj.1983.24.1.1

Abstract

In a previous report, it was felt that the rat tumor cell line, T-333, was a mixture of heterogeneous cells with different characteristics with respect to karyotype, tumorigenicity, and response to Rolls Sarcoma virus (RSV) infection. These characteristics of hetero-geneous cell subpopulations could be selected by use of different culture substrates. In this experiment, diversity of the cells in response to complement mediated cytolysis employing syngeneic rat anti-sera was studied. More than 50% of the glass grown cells were lysed while only 19% of the plastic grown cells were lysed by the specific immune sera of syngeneic rats. This finding suggests that growth in plastic culture wares selects cells with resistance to complement mediated cytolysis. It seems likely that the previously reported enhanced tumorigenicity of plastic grown T-333 cells is due to clonal selection of cell subpopulations which can better tolerate at least one arm of the in vivo immune surveillance system.

Keyword

in vitro; in plastico; ploidy; microcytotoxicity; heterogeneity; tumorigenicity; immunogenicity

MeSH Terms

Animal
Cells, Cultured
Cytotoxicity, Immunologic
Neoplasms, Experimental/immunology*
Plastics
Rats
Rats, Inbred Strains