J Korean Med Sci.  1998 Jun;13(3):311-316. 10.3346/jkms.1998.13.3.311.

Loss of heterozygosity on chromosome XP22.2-p22.13 and Xq26.1-q27.1 in human breast carcinomas

• Choi C
• Kim MH
• Juhng SW

• KMID: 1745556
• DOI: http://doi.org/10.3346/jkms.1998.13.3.311

Abstract

In an attempt to investigate the X chromosome harboring putative tumor suppressor genes (TSGs) in sporadic breast carcinoma, we performed loss of heterozygosity (LOH) studies on 23 breast carcinomas using 15 polymorphic markers covering the whole X chromosomes. Matched DNA extracted from tumor samples and corresponding normal tissues were analyzed by polymerase chain reactions (PCR) using microsatellite markers. In 10 cases (43.5%), LOH was detected for at least 1 of the 15 polymorphic markers of the X chromosome tested. Four cases carried a LOH at Xp, and three cases LOH on Xp and Xq. Three cases carried a LOH Xq. Percentage of LOH was relatively high in DXS987 (26.7%), DXS999(30.0%), HPRT(21.4%), DXS1062(23.1%) loci. Common regions of deletions were found on Xp22.2-p22.13 (30% of LOH) measuring about 4.5Mb and Xq26.1-q27.1 (23.1% of LOH) measuring 10 Mb. The deleted allele was an active copy of the X chromosome. The results indicate the TSGs on the X chromosome are involved in breast cancer.

MeSH Terms

Adenocarcinoma/genetics*
Adult
Alleles
Breast Neoplasms/genetics*
DNA, Neoplasm/genetics
Female
Genes, Suppressor, Tumor
Human
Loss of Heterozygosity*
Middle Age
Polymorphism (Genetics)
X Chromosome*