Clin Exp Reprod Med.  2012 Jun;39(2):81-86. 10.5653/cerm.2012.39.2.81.

Prooxidant-antioxidant balance and malondialdehyde over time in adult rats after tubal sterilization and vasectomy

• Faramarzi
• Seifi B
• Sadeghipour HR
• Shabanzadeh A
• Ebrahimpoor M

• KMID: 1743695
• DOI: http://doi.org/10.5653/cerm.2012.39.2.81

Abstract

OBJECTIVE
Sterilization (tubal sterilization and vasectomy) is a widely applied contraceptive method worldwide. Although most studies have described sterilization as a safe method, there are reports of tubal ligation (TL) and vasectomy complications. The aim of this study was to evaluate the effects of TL and vasectomy on the serum oxidative stress, specifically prooxidant-antioxidant balance (PAB) and malondialdehyde (MDA) levels, over time.
METHODS
Male and female rats were classified into vasectomy, sham-vasectomy, TL, and sham-TL groups, respectively. The PAB and MDA levels were measured on days 15 and 45 and months 3 and 6 after the intervention. For female rats, blood sampling was performed during the diestrous phase and estradiol and progesterone were also measured.
RESULTS
Serum PAB and MDA increased after TL (p<0.05). Vasectomy increased serum MDA remarkably after 45 days, 3 months, and 6 months (p<0.05). After vasectomy, serum PAB also increased although not significantly. Serum estradiol and progesterone decreased remarkably in the TL group compared to the sham group (p<0.05).
CONCLUSION
Bilateral TL and vasectomy both increase the serum oxidative stress; however the imbalance after TL was very noticeable. As for the TL, the reduction of serum estrogen levels can be involved in this imbalance. Complications followed by TL or vasectomy could be due to increased levels of oxidants. Thus, prescribing antioxidants during and or after surgery may be a solution.

Keyword

Tubal ligation; Vasectomy; Pro-oxidant-antioxidant balance; Malondialdehyde

MeSH Terms

Adult
Animals
Antioxidants
Contraception
Estradiol
Estrogens
Female
Humans
Male
Malondialdehyde
Oxidants
Oxidative Stress
Progesterone
Rats
Salicylamides
Sterilization
Sterilization, Tubal
Vasectomy
Antioxidants
Estradiol
Estrogens
Malondialdehyde
Oxidants
Progesterone
Salicylamides