Korean J Ophthalmol.  1991 Jun;5(1):1-8. 10.3341/kjo.1991.5.1.1.

Fibronectin in the trabecular meshwork: immunofluorescent and immunoelectron microscopic findings

• Choi YI
• Youn DH
• Ko MK
• Choe JK

• KMID: 1735071
• DOI: http://doi.org/10.3341/kjo.1991.5.1.1

Abstract

We examined the fibronectin (FN) secretion of cultured trabecular meshwork (TM) cells in a normal human eye by indirect immunofluorescent technique using mouse anti-human FN monoclonal antibody and FITC-conjugated goat anti-mouse IgG. To localize FN on frozen sections of normal TM, which were obtained from 7 enucleated eyes owing to traumatic eyeball rupture, the same indirect immunofluorescent method was used. Immunoelectron microscopy was applied to demonstrate the distribution pattern of FN in the normal TM of 2 human eyes using an avidin-biotin-peroxidase complex method. In the tissue culture of TM, the TM cell walls and extracellular matrices showed an intense staining with antibody to FN. Indirect immunofluorescent staining of FN on frozen sections of TM showed strong positive reactions in the subendothelial region. There was no reaction in the central core of the trabecular beam. Immunoelectron microscopy revealed the reaction products to FN in the areas lining the trabecular endothelial cells.

Keyword

fibronectin; trabecular meshwork; tissue culture; indirect immunof-luorescehce; irnrnunoeleclron microscopy

MeSH Terms

Adolescent
Adult
Antibodies, Monoclonal
Cells, Cultured
Fibronectins/biosynthesis/*metabolism
Fluorescent Antibody Technique
Humans
Male
Microscopy, Immunoelectron
Trabecular Meshwork/*metabolism/ultrastructure
Antibodies, Monoclonal
Fibronectins