J Korean Med Sci.  2000 Feb;15(1):53-58. 10.3346/jkms.2000.15.1.53.

Mutations of hepatitis C virus 1b NS5A 2209-2248 amino acid sequence is not a edictive factor for response to interferon-alpha therapy and development of patocellular carcinoma

• Bae SH
• Park YM
• Yoo DG
• Choi JY
• Byun BH
• Yang JM
• Lee CD
• Cha SB
• Park DH
• KiM BS

• KMID: 1731234
• DOI: http://doi.org/10.3346/jkms.2000.15.1.53

Abstract

Genetic changes between codons 2209 and 2248 of NS5A of genotype 1b hepatitis C virus (HCV-1b) have been reported to be associated with the sensitivity to interferon-alpha (IFN-alpha). The present study was performed to analyze such relationship in Korean patients with chronic hepatitis C and HCV-1b (n=19), including 12 chronic hepatitis C patients treated with IFN-alpha, 3 chronic hepatitis C patients without treatment as controls, and 4 patients with hepatocellular carcinoma (HCC). Two serum samples, before and after the treatment, were analyzed for the mutations by reverse transcription-polymerase chain reaction, cloning and sequencing. The mutations were identified in 32% (6/19), including five intermediate type (1-3 mutations) and one mutant type (4 or more). In 12 patients treated with IFN-alpha, the number of amino acid substitutions in NS5A2209-2248 was not associated with outcome of the treatment.

Keyword

Hepatitis C Chronic; Genotype; Viral Nonstructural Proteins; Interferon-Alpha; Carcinoma; hepatocellular

MeSH Terms

Adult
Aged
Amino Acid Sequence
Antiviral Agents/therapeutic use*
Base Sequence
Carcinoma, Hepatocellular/virology*
Carcinoma, Hepatocellular/blood
Codon
Female
Genotype
Hepatitis C, Chronic/virology*
Hepatitis C, Chronic/drug therapy*
Hepatitis C, Chronic/blood
Hepatitis C-Like Viruses/isolation & purification
Hepatitis C-Like Viruses/genetics*
Hepatitis C-Like Viruses/classification
Human
Interferon-alpha/therapeutic use*
Liver Neoplasms/virology*
Liver Neoplasms/blood
Male
Middle Age
Molecular Sequence Data
Mutation*
Prognosis
Reverse Transcriptase Polymerase Chain Reaction