J Korean Med Sci.  2000 Aug;15(4):452-456. 10.3346/jkms.2000.15.4.452.

The effect of isochromosome 17q presence, proliferative and apoptotic indices, expression of c-erbB-2, bcl-2 and p53 proteins on the prognosis of medulloblastoma

• Nam DH
• Wang KC
• Kim YM
• Chi JG
• Kim SK
• Cho BK

• KMID: 1730644
• DOI: http://doi.org/10.3346/jkms.2000.15.4.452

Abstract

Medulloblastoma accounts for 20 to 25+ACU- of all intracranial neoplasms in children. The significance of the presence of isochromosome 17q (i(17q)), proliferative potential, apoptotic activity, and expression of c-erbB-2, bd-2, and p53 proteins in predicting long-term survival of patients with medulloblastomas was investigated. Twenty children were divided into two groups (favorable and poor outcome groups). Ten children with favorable outcome (FO) were disease-free during the follow-up period (median: 61.5 months). The other ten children with poor outcome (PO) died of disease progression, having a median survival of 18 months. Fluorescent in situ hybridization (FISH) for i(17q), terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and immunohistochemistry for Ki-67, c-erbB-2, bcl-2, and p53 proteins was performed in these patients. Nine out of 17 children showed i(17q). There was no difference in the rate of positive i(17q) between the FO and PO groups. The presence of i(17q) was not significantly related to biological factors that we investigated. Unlike the prominent presence of the proliferative potential and p53 expression in children with PO, apoptotic activity and expression of c-erbB-2 and bcl-2 had no correlation with the outcome.

Keyword

Medulloblastoma; Chromosomes, Human, Pair17; Ki-67 antigen; Apoptosis; Protein p53; Prognosis

MeSH Terms

Adolescence
Apoptosis
Brain Neoplasms/pathology
Brain Neoplasms/mortality
Brain Neoplasms/genetics+ACo-
Cell Division
Child
Child, Preschool
Chromosomes, Human, Pair 17/ultrastructure+ACo-
Chromosomes, Human, Pair 17/genetics
Comparative Study
Disease-Free Survival
Female
Follow-Up Studies
Genes, bcl-2+ACo-
Genes, erbB-2+ACo-
Genes, p53+ACo-
Human
In Situ Hybridization, Fluorescence
In Situ Nick-End Labeling
Infant
Ki-67 Antigen/analysis
Male
Medulloblastoma/pathology
Medulloblastoma/mortality
Medulloblastoma/genetics+ACo-
Neoplasm Proteins/analysis
Prognosis
Retrospective Studies
Survival Analysis
Treatment Outcome