Korean J Parasitol.  2005 Dec;43(4):149-156. 10.3347/kjp.2005.43.4.149.

Expressed sequence tags analysis of Blattella germanica

Affiliations
  • 1Department of Otorhinolaryngology, Kosin University College of Medicine, Busan 602-703, Korea.
  • 2Department of Parasitology, Kosin University College of Medicine, Busan 602-703, Korea. sunnyock@kosin.ac.kr
  • 3Department of Parasitology, Pusan National University College of Medicine, Busan, 602-739, Korea.

Abstract

Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

Keyword

Blattella germanica; cDNA library; ESTs; BLAST search; novel genes

MeSH Terms

Sequence Alignment
Reverse Transcriptase Polymerase Chain Reaction
Molecular Sequence Data
Male
Female
*Expressed Sequence Tags
Blattellidae/*genetics
Base Sequence
Animals
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