Korean J Parasitol.  2005 Mar;43(1):27-32. 10.3347/kjp.2005.43.1.27.

Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii

• Lin A
• Shin EH
• Kim TY
• Park JH
• Guk SM
• Chai JY

• KMID: 1720496
• DOI: http://doi.org/10.3347/kjp.2005.43.1.27

Abstract

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.

Keyword

Toxoplasma gondii; Korean isolate-1 (KI-1); PCR-RFLP; sequence

MeSH Terms

Amino Acid Sequence
Animals
Antigens, Protozoan/*genetics
Base Sequence
Genes, Protozoan
Genotype
Polymorphism, Genetic
RNA, Protozoan
Research Support, Non-U.S. Gov't
Toxoplasma/*genetics