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Identification of Atg8 Isoform in Encysting Acanthamoeba

Moon EK, Hong Y, Chung DI, Kong HH

  • KMID: 1792139
  • Korean J Parasitol.
  • 2013 Oct;51(5):497-502.
Autophagy-related protein 8 (Atg8) is an essential component of autophagy formation and encystment of cyst-forming parasites, and some protozoa, such as, Acanthamoeba, Entamoeba, and Dictyostelium, have been reported to possess...
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Subgenus classification of Acanthamoeba by riboprinting

Chung DI, Yu HS, Hwang MY, Kim TH, Kim TO, Yun HC, Kong HH

Subgenus classification of Acanthamoeba remains uncertain. Twenty-three reference strains of Acanthamoeba including 18 (neo)type-strains were subjected for classification at the subgenus level by riboprinting. PCR/RFLP analysis of 18S rRNA gene...
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Species identification and molecular characterization of Acanthamoeba isolated from contact lens paraphernalia

Lee SM, Choi YJ, Ryu HW, Kong HH, Chung DI

We applied ribosomal RNA gene (rDNA) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to identify Acanthamoeba isolates from contact lens paraphernalia, and characterized these on the basis of mitochondrial DNA...
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Molecular Characterization of Various Trichomonad Species Isolated from Humans and Related Mammals in Indonesia

Kamaruddin M, Tokoro M, Rahman MM, Arayama S, Hidayati A, Syafruddin D, Asih PB, Yoshikawa , Kawahara E

Trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. Twenty-one strains of trichomonads isolated from...
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Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya)

Chae JS, Kwon OD, Holman PJ, Waghela SD, Wagner GG, Lee JM

Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine Theileria isolates from Korea (KLS and KCB) and Japan (JHS) were determined. The genes from each isolate were amplified...
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PCR Detection and Molecular Characterization of Pentatrichomonas hominis from Feces of Dogs with Diarrhea in the Republic of Korea

Kim YA, Kim HY, Cho SH, Cheun HI, Yu JR, Lee SE

Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce...
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Expression of Exogenous Human Hepatic Nuclear Factor-1alpha by a Lentiviral Vector and Its Interactions with Plasmodium falciparum Subtilisin-Like Protease 2

Liao S, Liu Y, Zheng B, Cho PY, Song HO, Lee YS, Jung SY, Park H

The onset, severity, and ultimate outcome of malaria infection are influenced by parasite-expressed virulence factors as well as by individual host responses to these determinants. In both humans and mice,...
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Microarray Analysis of Differentially Expressed Genes between Cysts and Trophozoites of Acanthamoeba castellanii

Moon EK, Xuan YH, Chung DI, Hong Y, Kong HH

Acanthamoeba infection is difficult to treat because of the resistance property of Acanthamoeba cyst against the host immune system, diverse antibiotics, and therapeutic agents. To identify encystation mediating factors of...
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Genetic characteristics of the Korean isolate KI-1 of Toxoplasma gondii

Lin A, Shin EH, Kim TY, Park JH, Guk SM, Chai JY

Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by...
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cDNAs encoding the antigenic proteins in pathogenic strain of Entamoeba histolytica

Im KI, Choi JT, Hong YP, Kim TE, La MS

The differential display reverse transcription polymerase chain reaction (DDRT-PCR) analysis was performed to identify the pathogenic strain specific amplicons. mRNAs were purified from the trophozoites of the...
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Presence of Cryptosporidium spp. and Giardia duodenalis in Drinking Water Samples in the North of Portugal

Almeida A, Moreira MJ, Soares S, Delgado MD, Figueiredo J, Silva E, Castro A, Cosa JM

Cryptosporidium and Giardia are 2 protozoan parasites responsible for waterborne diseases outbreaks worldwide. In order to assess the prevalence of these protozoans in drinking water samples in the northern part...
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A riboprinting scheme for identification of unknown Acanthamoeba isolates at species level

Kong HH, Chung DI

We describe a riboprinting scheme for identification of unknown Acanthamoeba isolates at the species level. It involves use of the PCR-RFLP of small subunit ribosomal RNA gene (riboprint) of...
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