Korean J Parasitol.
2002 Jun;40(2):89-92. 10.3347/kjp.2002.40.2.89.
Excretory bladder: the source of cysteine proteases in Paragonimus westermani metacercariae
- Affiliations
-
- 1Department of Parasitology, College of Medicine, Cheju National University, Jeju, Korea.
- 2Department of Parasitology, College of Medicine, Chung-Ang University, Seoul, Korea.
- 3Department of Molecular Parasitology, Sungkyunkwan University School of Medicine, Suwon, Korea.
- KMID: 1720410
- DOI: http://doi.org/10.3347/kjp.2002.40.2.89
Abstract
- The cysteine proteases of Paragonimus westermani metacercariae are involved in metacercarial excystment, host immune modulation, and possibly in tissue penetration. In order to clarify the origin of the enzymes, 28 and 27 kDa cysteine proteases in metacercarial excretory-secretory products were purified through the FPLC system using Mono Q column chromatography. The polyclonal antibodies to the enzymes were produced in BALB/c mice. Immunolocalization studies revealed that both cysteine proteases were distributed at the linings of excretory bladder and excretory concretions of the metacercariae. It was suggested that the excretory epithelium of P. westermani undertake the secretory function of metacercarial cysteine proteases, in addition to its role as a route for eliminating waste products.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Activities of different cysteine proteases of Paragonimus westermani in cleaving human IgG
- Excretory-secretory product of Paragonimus westermani newly excysted metacercariae inhibits superoxide production of granulocytes stimulated with IgG
- Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease
- A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils
- A new second intermediat host of genus Paragonimus
