Korean J Parasitol.  2011 Sep;49(3):291-294. 10.3347/kjp.2011.49.3.291.

NF-kappaB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products

Affiliations
  • 1Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea. myeong@yuhs.ac
  • 2Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
  • 3Brain Korea 21 for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.

Abstract

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1x10(7) live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-kappaB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with ikappaB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-kappaB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

Keyword

Trichomonas vaginalis; human neutrophil; IL-8; NF-kappaB; CREB
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