Korean J Parasitol.  2011 Sep;49(3):291-294. 10.3347/kjp.2011.49.3.291.

NF-kappaB and CREB Are Involved in IL-8 Production of Human Neutrophils Induced by Trichomonas vaginalis-Derived Secretory Products

• Nam YH
• Min D
• Park SJ
• Kim KA
• Lee YA
• Shin MH

• KMID: 1715654
• DOI: http://doi.org/10.3347/kjp.2011.49.3.291

Abstract

Trichomonas vaginalis is a flagellated lumen-dwelling extracellular protozoan parasite that causes human trichomoniasis via sexual intercourse. Human neutrophils play a crucial role in acute tissue inflammatory responses in T. vaginalis infection. In this study, we investigated the signaling mechanism of neutrophil responses when stimulated with T. vaginalis-derived secretory products (TvSP), which were collected from 1x10(7) live trichomonads. Incubation of human neutrophils isolated from peripheral blood with TvSP induced up-regulation of IL-8 protein secretion. In addition, stimulation with TvSP induced phosphorylation of NF-kappaB and CREB in neutrophils. Moreover, TvSP-induced IL-8 production was also significantly inhibited by pretreatment of neutrophils with ikappaB inhibitor or CREB inhibitor. These results suggest that transcription factors NF-kappaB and CREB are involved in IL-8 production in human neutrophils induced by stimulation with T. vaginalis infection.

Keyword

Trichomonas vaginalis; human neutrophil; IL-8; NF-kappaB; CREB

MeSH Terms

Cyclic AMP Response Element-Binding Protein/*metabolism
Human Experimentation
Humans
Interleukin-8/*metabolism
Male
NF-kappa B/*metabolism
Neutrophils/*immunology
Phosphorylation
Trichomonas vaginalis/*immunology