Korean J Parasitol.
2011 Mar;49(1):85-90. 10.3347/kjp.2011.49.1.85.
CD8+ T-cell Activation in Mice Injected with a Plasmid DNA Vaccine Encoding AMA-1 of the Reemerging Korean Plasmodium vivax
- Affiliations
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- 1Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Disease, Seoul National University Medical Research Center, Seoul 110-799, Korea. cjy@snu.ac.kr
- 2Department of Environmental Medical Biology, Yonsei University College of Medicine, Seoul 120-752, Korea.
- 3Department of Anatomy, College of Medicine, Korea University, Brain Korea 21, Seoul 136-705, Korea.
- 4Divison of Infection and Immunology, Graduate School of Medicine, Korea University, Seoul 136-701, Korea.
- 5Department of Parasitology, Graduate School of Medicine, Gunma University, Maebashi 371-8581, Japan.
- 6Department of Parasitology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
- 7Seoul National University Bundang Hospital, Seongnam 463-707, Korea.
- KMID: 1715639
- DOI: http://doi.org/10.3347/kjp.2011.49.1.85
Abstract
- Relatively little has been studied on the AMA-1 vaccine against Plasmodium vivax and on the plasmid DNA vaccine encoding P. vivax AMA-1 (PvAMA-1). In the present study, a plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax has been constructed and a preliminary study was done on its cellular immunogenicity to recipient BALB/c mice. The PvAMA-1 gene was cloned and expressed in the plasmid vector UBpcAMA-1, and a protein band of approximately 56.8 kDa was obtained from the transfected COS7 cells. BALB/c mice were immunized intramuscularly or using a gene gun 4 times with the vaccine, and the proportions of splenic T-cell subsets were examined by fluorocytometry at week 2 after the last injection. The spleen cells from intramuscularly injected mice revealed no significant changes in the proportions of CD8+ T-cells and CD4+ T-cells. However, in mice immunized using a gene gun, significantly higher (P<0.05) proportions of CD8+ cells were observed compared to UB vector-injected control mice. The results indicated that cellular immunogenicity of the plasmid DNA vaccine encoding AMA-1 of the reemerging Korean P. vivax was weak when it was injected intramuscularly; however, a promising effect was observed using the gene gun injection technique.
MeSH Terms
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Animals
Antigens, Protozoan/administration & dosage/genetics/*immunology
CD8-Positive T-Lymphocytes/*immunology
COS Cells
Cercopithecus aethiops
Humans
Lymphocyte Activation
Malaria, Vivax/*immunology/parasitology
Membrane Proteins/administration & dosage/genetics/*immunology
Mice
Mice, Inbred BALB C
Plasmodium vivax/genetics/*immunology
Protozoan Proteins/administration & dosage/genetics/*immunology
Protozoan Vaccines/administration & dosage/genetics/*immunology
Vaccines, DNA/administration & dosage/genetics/*immunology
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