Korean J Parasitol.  1996 Sep;34(3):211-213. 10.3347/kjp.1996.34.3.211.

Electrophoretic variations of enzyme, GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4) in characterizing clones and isolates of Malaysian Plasmodium falciparum

Affiliations
  • 1School of Pharmaceutical Sciences, University of Science Malaysia, Minden, Filaria, Malaysia.

Abstract

: Malaysian, African and Thai Plasmodium falciparum isolates were cultured in vitro by the Trager and Jensen method (1976; 1977) and were later cloned by the limiting dilution method (Rosario, 1981). Forty- eight clones were obtained and were characterized by electrophoretic variations of GDH (NADP-dependent glutamate dehydrogenase)(EC. 1.4.1.4). It was found that they were pure clones because they possessed either GDH-1 or GDH-2 unlike their parent isolates which exhibited both GDH-1 and GDH-2.


MeSH Terms

Animal
Electrophoresis
Genotype
Glutamate Dehydrogenase (NADP+)/*ISOLATION, PURIFICATION/CLASSIFICATION
Isoenzymes/*ISOLATION, PURIFICATION/CLASSIFICATION
Plasmodium falciparum/GENETICS/*ENZYMOLOGY
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