J Korean Med Sci.  2007 Oct;22(5):825-831. 10.3346/jkms.2007.22.5.825.

TAP1 and TAP2 Gene Polymorphisms in Korean Patients with Allergic Rhinitis

• Kim KR
• Cho SH
• Choi SJ
• Jeong JH
• Lee SH
• Park CW
• Tae K

• KMID: 1713288
• DOI: http://doi.org/10.3346/jkms.2007.22.5.825

Abstract

Antigen peptides are actively transported across the endoplasmic reticulum by the transporters associated with antigen presentation (TAP). TAP genes polymorphism could influence the selection process that determines which antigen peptides play a role in the pathogenesis of allergic rhinitis. The aim of this study was to investigate the association of TAP genes polymorphism with allergic rhinitis. TAP1 and TAP2 genotyping were performed on 110 allergic rhinitis patients and 107 healthy controls. TAP1 polymorphic residues at codons 333 and 637, and TAP2 polymorphic residues at codons 379, 565, 651, and 665 were analyzed by the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR). Analysis of TAP1 gene polymorphism demonstrated decreased frequencies of Ile/Val genotype at codon 333, Asp/Gly genotype at codon 637, and haplotype A and B in allergic rhinitis patients when compared to controls (p<0.05). However, there was no significant difference in the genotype, phenotype, or allele frequencies at four TAP2 codons between controls and allergic rhinitis patients. In conclusion, TAP1 gene polymorphism may be an important factor contributing to the genetic susceptibility in the development of allergic rhinitis in the Korean population.

Keyword

Allergic Rhinitis; TAP1; TAP2; Polymorphism

MeSH Terms

ATP-Binding Cassette Transporters/*genetics
Adolescent
Adult
Aged
Child
Codon
Female
Genetic Predisposition to Disease
Genotype
Humans
Hypersensitivity/*genetics
Hypersensitivity, Immediate/*genetics
Korea
Male
Middle Aged
*Polymorphism, Genetic
Rhinitis/*genetics

Figure

• 

  Fig. 1 TAP1 codon 333 genotyping by ARMS-PCR. Four oligonucleotides were included in each polymerase chain reaction mix. Lanes 4, 6, 7, 8 and 11 consist of one control and one allele-specific 241 bp fragment defining the Ile/Ile homozygote. Lanes 1, 2, 3, 9 and 10 consist of one control and two allele-specific, smaller fragments (241 bp and 351 bp) defining the Ile/Val heterozygote. Lane 5 consists of one control and one allele-specific 351 bp fragment defining the Val/Val homozygote.

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