J Korean Med Sci.  2007 Oct;22(5):810-814. 10.3346/jkms.2007.22.5.810.

Association of Polymorphisms in Monocyte Chemoattractant Protein-1 Promoter with Diabetic Kidney Failure in Korean Patients with Type 2 Diabetes Mellitus

• Moon JY
• Jeong L
• Lee S
• Jeong K
• Lee T
• Ihm CG
• Suh J
• Kim J
• Jung YY
• Chung JH

• KMID: 1713285
• DOI: http://doi.org/10.3346/jkms.2007.22.5.810

Abstract

Monocyte chemoattractant protein-1 (MCP-1) is suggested to be involved in the progression of diabetic nephropathy. We investigated the association of the -2518 A/G polymorphism in the MCP-1 gene with progressive kidney failure in Korean patients with type 2 diabetes mellitus (DM). We investigated -2518 A/G polymorphism of the MCP-1 gene in type 2 DM patients with progressive kidney failure (n=112) compared with matched type 2 DM patients without nephropathy (diabetic control, n=112) and healthy controls (n=230). The overall genotypic distribution of -2518 A/G in the MCP-1 gene was not different in patients with type 2 DM compared to healthy controls. Although the genotype was not significantly different between the patients with kidney failure and the diabetic control (p=0.07), the A allele was more frequent in patients with kidney failure than in DM controls (42.0 vs. 32.1%, p=0.03). The carriage of A allele was significantly associated with kidney failure (68.8 vs. 54.5%, OR 1.84, 95% CI 1.07-3.18). In logistic regression analysis, carriage of A allele retained a significant association with diabetic kidney failure. Our result shows that the -2518 A allele of the MCP-1 gene is associated with kidney failure in Korean patients with type 2 DM.

Keyword

Diabetic Nephropathies; Chemokine CCL2; Polymorphism

MeSH Terms

Adult
Aged
Alleles
Chemokine CCL2/*metabolism
Diabetes Mellitus, Type 2/ethnology/*genetics/*metabolism
Diabetic Nephropathies/ethnology/*genetics/*metabolism
Female
Genotype
Humans
Kidney Failure
Korea
Male
Middle Aged
*Polymorphism, Genetic
*Promoter Regions, Genetic
Risk Factors

Figure

• 

  Fig. 1 Representative 1% agarose gel stained with ethidium bromide and photographed under ultraviolet transillumination after PCR and digestion by Pvu II for MCP-1 genotyping. The upper band of 929 bp is the A allele and the lower band of 707 bp is the G allele (arrowheads). The A/A type is shown as a single upper band, the G/G type as a single lower band, and the G/A type as double bands.

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