Int J Oral Biol.  2013 Dec;38(4):169-173.

Fast temporal detection of intracellular hydrogen peroxide by HyPer

Affiliations
  • 1Department of Oral Biology, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 120-752, Korea. dmshin@yuhs.ac

Abstract

HyPer is the genetically encoded biosensor of intracellular hydrogen peroxide (H2O2), the most stable of the reactive oxygen species (ROS) generated by living cells. HyPer has a high sensitivity and specificity for detecting intracellular H2O2 by confocal laser microscopy. However, it was not known whether high speed ratiometric imaging of H2O2 by HyPer is possible. We thus investigated the sensitivity of HyPer in detecting changes to the intracellular H2O2 levels in HEK293 and PC12 cells using a microfluorometer imaging system. Increase in the HyPer ratio were clearly evident on stimulations of more than 100 microM H2O2 and fast changes in the HyPer ratio were observed on ratiometric fluorescent images after H2O2 treatment. These results suggest that HyPer is a potent biosensor of the fast temporal production of intracellular H2O2.

Keyword

HyPer; H2O2; ROS; high speed detection

MeSH Terms

Animals
Biosensing Techniques
Hydrogen Peroxide*
Hydrogen*
Microscopy, Confocal
PC12 Cells
Reactive Oxygen Species
Sensitivity and Specificity
Hydrogen
Hydrogen Peroxide
Reactive Oxygen Species
Full Text Links
  • IJOB
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2022 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr