Ann Lab Med.  2013 Jul;33(4):300-303. 10.3343/alm.2013.33.4.300.

Discrepant Results for BRAF V600E Mutation Among Different Specimens in a Case of Hairy Cell Leukemia

• Moon HW
• Hur M
• Han SH
• Yun YM
• Kim SY

• KMID: 1707241
• DOI: http://doi.org/10.3343/alm.2013.33.4.300

Abstract

No abstract available.

MeSH Terms

Antineoplastic Agents/therapeutic use
Bone Marrow Cells/pathology
Cladribine/therapeutic use
DNA Mutational Analysis
Female
Humans
Immunophenotyping
Leukemia, Hairy Cell/*diagnosis/drug therapy/*genetics
Middle Aged
*Mutation
Proto-Oncogene Proteins B-raf/*genetics
Reticulin/metabolism
Antineoplastic Agents
Cladribine
Proto-Oncogene Proteins B-raf
Reticulin

Figure

• 

  Fig. 1 (A) Bone marrow aspirate smear showing characteristic hairy cells with cytoplasmic projections and frayed cytoplasmic border (Wright-Giemsa stain, ×1,000). (B) Bone marrow biopsy section showing diffuse solid infiltration of medium-sized lymphoid cells with abundant cytoplasm (fried-egg appearance) (H&E stain, ×400). (C) Extensive reticulin fibrosis (Reticulin stain, ×400). (D) Strong positivity for CD20 (CD 20 immunostain, ×200).

• 

  Fig. 2 Immunophenotyping of bone marrow aspirate shows abnormal lymphoid cells positive for CD19, CD20, and CD11c and negative for CD5 and CD10. HLA-DR, cytoplasmic CD79a, and CD2 were also positive, and other T-lymphoid (CD7, CD3, and cCD3) and myeloid markers (CD13, CD33, myeloperoxidase and CD14) were negative (not shown).

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