J Vet Sci.  2013 Sep;14(3):249-256. 10.4142/jvs.2013.14.3.249.

Effects of administration of IH901, a ginsenoside intestinal metabolite, on muscular and pulmonary antioxidant functions after eccentric exercise

• Lee NJ
• Lee JW
• Sung JH
• Ko YG
• Hwang S
• Kang JK

• KMID: 1705554
• DOI: http://doi.org/10.4142/jvs.2013.14.3.249

Abstract

This study was conducted to investigate whether administration of IH901, a ginseng intestinal metabolite, ameliorates exercise-induced oxidative stress while preserving antioxidant defense capability in rat skeletal muscles and lung. Eight adult male Sprague-Dawley rats per group were randomly assigned to the resting control, exercise control, resting with IH901 (25, 50, and 100 mg/kg) consumption (R/IH901), or exercise with IH901 (25, 50, and 100 mg/kg) consumption (E/IH901) group. The trained groups ran 35 min 2 days/week for 8 weeks. To analyze the IH901-training interaction, serum biochemical analysis, lipid peroxidation, citrate synthase, protein oxidation, antioxidant and superoxide dismutase in skeletal muscles and lung tissue were measured. Compared to the exercise control group, animals that consumed IH901 had significantly increased exercise endurance times (p < 0.05) and decreased plasma creatine kinase and lactate dehydrogenase levels (p < 0.05), while those in the E/IH901 groups had increased citrate synthase and anti-oxidant enzymes and decreased lipid peroxidation and protein oxidation (p < 0.05). In conclusion, IH901 consumption in aging rats after eccentric exercise has beneficial effects on anti-inflammatory and anti-oxidant activities through down-regulation of pro-inflammatory mediators, lipid peroxidation, and protein oxidation and up-regulation of anti-oxidant enzymes.

Keyword

anti-oxidant activity; eccentric exercise; IH901; lipid peroxidation; rat

MeSH Terms

Aging
Animals
Antioxidants/administration & dosage/*pharmacology
Dose-Response Relationship, Drug
Lung/*drug effects/metabolism
Male
Muscle, Skeletal/*drug effects/metabolism
Oxidative Stress/*drug effects
Panax/chemistry
Physical Conditioning, Animal
Rats
Rats, Sprague-Dawley
Sapogenins/administration & dosage/blood/*metabolism/*pharmacology
Specific Pathogen-Free Organisms
Antioxidants
Sapogenins

Figure

• 

  Fig. 1 The endurance time of treadmill running to exhaustion in rats treated with vehicle or IH901. Values are expressed as the mean ± SD (n = 8/group). The rats performed a graded treadmill run to fatigue on a customized rodent treadmill. The protocol consisted of having rats run upward at a speed of 25 cm/sec (0~2 weeks); 30 cm/sec (2~4 weeks) and 35 cm/sec (4~8 weeks). EC: exercise control. *p < 0.05 (vs. EC).

• 

  Fig. 2 Plasma creatine kinase (CK) and lactate dehydrogenase (LDH) concentrations after eccentric exercise. Values are expressed as the mean ± SD (n = 8/group). The rats performed a graded treadmill run to fatigue on a customized rodent treadmill. The protocol consisted of having rats run upward at a speed of 25 cm/sec (0~2 weeks); 30 cm/sec (2~4 weeks) and 35 cm/sec (4~8 weeks). Blood samples were collected before exercise (pre) and at 30 min after exercise (after). RC: resting control. *p < 0.05 (vs. EC), ‡p < 0.05 (vs. RC).

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