Korean J Dermatol.  1976 Dec;14(4):297-304.

Effect of Topical Vitamin A Acid on Mouse Skin

• Kim HS

Abstract

These studies were undertaken not only to evaluate the morphological change of topical vitamin A acid but also to compare the methodological difference in application, simple application and ODT, on mature mouse tail skin. All 18 animals were divided into 5 groups and serial biopsy was done after application of 0. 05% vitamine A achid and ointment base. I. 1 untreated control mouse. II. 2 mice treated with simple application of ointment base for 7 days. III. 3 mice treated with ODT of ointment base for 7 days. IV. 8 mice treated with sirnple application of 0. 05% vitamine A acid for 21 days. V. 4 mice treated with ODT of 0. 05% vitamin A acid for 7 days. The results are as follows: 1. Increase in epidermal thickness (60. 2 micron) was produced by simple applic- ation of 0. 05 % vitamin A acid for 21 days, but epidermal hypoplasia was noticert by ODT for 7 days. 2. Induction of granular layer was demonstrated by simple application of 0. 05 % vitamin A acid for 21 days. 3. There was a tendency of horny layer desquamation and epidermal darnage in application of vitamine A acid. Most severe desquamation and damage was produced by simple application of 0. 05% vitarnine A acid for 7 days. After that recovery was noticed in spite of continuous application for 21 days. 4. Epidermal hyperplasia, slight detachrnent of horny layer and epidermal damage were also produced by ODT of ointment base, probably due to nonspecific stimulation.

MeSH Terms

Animals
Biopsy
Hyperplasia
Mice*
Skin*
Tail
Tretinoin*
Vitamin A*
Vitamins*
Tretinoin
Vitamin A
Vitamins