Korean J Dermatol.
1960 Dec;1(2):1-18.
The Influence of Aicdosis and Alkalosis upon the Allergic Changes of the Urinary Bladder
Abstract
- Experimental materials were healthy rabbits weighing around 2Kgs and were fed with bean curd through the experimental period. The rabbits used in these experiments were divided into three groups; Normal group, Acidosis group and Alkalosis group, each of these groups were also divided into two groups; Sensitized group and Non-sensitized group. In the normal group, the rabbits were fed with ordinary food. In thc second group, for producing acidosis, 5gms of white sugar per Kg of body weight mixing with ordinary food was given for 60 days. In the third group, for the sake of Alkalosis, 0.1 gm of calcium chloride per Kg of body weight mixing with ordinary food was added for 60 days. For sensitizing, 1cc of horse serum per Kg. of body weight was injected into the subcutaneous tissue of the abdominal wall, 4times every other day. Non-sensitizing groups-These groups were not sensitized and were used as the controll, A single injection of normal horse serum O. Icc per Kg. of body weight was injected into the subserosa of thc trigonum vesicae. Sensitized groups-Two weeks after the sensitization, reinjection was carried out in the same manner used in the non-sensitized groups. For the determination of acidosis and alkalosis, the CO2 combining power of the blood plasma of the whole rabbits were measured by Van Slyke's method. The CO2 combining power of the blood plasma in the Normal group showed the range of 42.0-50.5 Vol% in the Acidosis group showed 30.5-36.0 Vol%, and in the Alkalosis group, 52.0-68.5 VOl%. After the injection of horse serum into the urinary bladder wall. the rabbits were killed at the intervals of 3, 6, 12, 24, 48, 72, hrs and 1, 2 weeks by air embolism. And then the trigonum vesicae which were injected of horse scrum were excised and examined grossly and microscopically. For the histological examination, they were fixed in the 10% formalin. and stained by Haematoxylin-Eosin and Van Gieson'a methad. Results 1. Severe allergic changes occurred in the urinary bladder wall withthe reinjection of horse serum into the urinary bladder wall subserora of trigonum vesicae in the normal sensitized group. 2. More severe allergic changes were noted in the snsitized acidosis group than that in the normal sensitized group. 3. These allergic changes were milder in the sensitazed alkalosis group than that in the normal sensitized group. According to the above experiments, it was noted thc Acidosis group showed the most serious histological changes and proved that Acidosis influences the aggravation of allergic changes and Alkalosis somewhat reduces them in thc urinary bladder.
