Korean J Urol.  1996 Dec;37(12):1333-1338.

Effect of Tumor Necrosis Factor-alpha Gene Transduced Bladder Tumor Cell Xenograft on Survival of C3H/He Mice

• Pyun SS
• Lee ES
• Lee C

Abstract

OBJECTIVE
To evaluate the effect of tumor necrosis factor-alpha (TNF-alpha)gene transduced bladder tumor cells on survival of C3H/He mice.
MATERIALS AND METHODS
The complete cDNA of TNF-alpha was introduced to mouse bladder tumor cell line(MBT-2) using a retroviral vector. Gene transfer and expression were confirmed by polymerase chain reaction(PCR) and reverse transcription polymerase chain reaction(RT-PCR). Produced TNF-alpha was measured by ELISA. TNF-alpha gene transduced and parental cells were xenografted in C3H/He mice. Implantation and growth rate and survival of experimental animals were analysed.
RESULTS
Successful gene transfer and expression were confirmed. TNF-G gene transduced MBT-2 cells secreted TNF-alpha at the concentration of 47.4pg/106cells/24hours. The implantation and growth rate of TNF-t gene transduced MBT-2 cells were much lower than those of parental cells and survival of experimental animals was different between TNF-t gene transduced MBT-2 and parental cells(P<0.05).
CONCLUSION
This study shows potential advantages of localized TNF-a secretion to induce potent antitumorigenic response. Tumor cell targeted TNF-a gene therapy could become a useful tool in treatment of bladder cancer.

Keyword

xenograft; tumor growth; gene therapy; tumor necrosis factor-alpha

MeSH Terms

Animals
DNA, Complementary
Enzyme-Linked Immunosorbent Assay
Genetic Therapy
Heterografts*
Humans
Mice*
Parents
Reverse Transcription
Tumor Necrosis Factor-alpha*
Urinary Bladder Neoplasms*
Urinary Bladder*
Zidovudine
DNA, Complementary
Tumor Necrosis Factor-alpha
Zidovudine