Korean J Med.  2003 Aug;65(2):196-204.

Immunohistochemical study of metalloproteinase-1 and tissue inhibitor of matrix metalloproteinase-1, -2 in the idiopathic interstitial pneumonia

• Kyung SY
• Lim YH
• An CH
• Park JW
• Jeong SH
• Shin EK
• Ha SY
• Lee JW

Abstract

BACKGROUND: In the idiopathic interstitial pneumonia (IIP), it has been known that imbalance between matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) is important factor for abnormal remodeling of lung parenchyme. This study was performed to compare the expression of MMPs and TIMPs in the normal subjects and patients with IIP.
METHODS
Seventeen patients were diagnosed as IIP by open lung biopsy (male: female 7:10) and four patients as normal control were diagnosed as lung cancer and treated by lobectomy or pneumonectomy from March, 1999 to August 2001 at Gil medical center. IIP group divided into UIP (n=10) and NSIP (n=7). MMP-1 and TIMP-2 of their lung tissue were stained by immunohistochemical method and were graded 4 levels (grage 0-3) following stained status.
RESULTS
MMP-1 was stained more strongly in the IIP than normal. But it had no differences between UIP and NSIP. TIMP-1 and-2 were stained more strongly in the UIP than NSIP but not stained in the normal. In the UIP, TIMP-2 was stained strongly in fibroblast foci.
CONCLUSION
These results suggst that imbalance between MMPs and TIMPs may be important factor of pathogenesis of pulmonary fibrosis in the IIP. It is thought that major site of TIMP-2 is myofibroblast in the fibroblast foci.

Keyword

Idiopathic intersitial pneumonia; Matrix Metalloproteinase; Tissue Inhibitor of metalloproteinase

MeSH Terms

Biopsy
Female
Fibroblasts
Humans
Idiopathic Interstitial Pneumonias*
Lung
Lung Neoplasms
Matrix Metalloproteinase 1*
Matrix Metalloproteinases
Metalloproteases
Myofibroblasts
Pneumonectomy
Pulmonary Fibrosis
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 1
Matrix Metalloproteinases
Metalloproteases
Tissue Inhibitor of Metalloproteinase-1
Tissue Inhibitor of Metalloproteinase-2