Korean J Phys Anthropol.
2004 Dec;17(4):351-362.
Characteristics of Mitochondrial Events in Synthetic Bile Acids-induced Apoptosis of Human Osteosarcoma Cells
- Affiliations
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- 1Department of Oral Anatomy, College of Dentistry, Pusan National University, Korea. parkbs@pusan.ac.kr
- 2Department of Anatomy, College of Medicine, Catholic University of Daegu, Korea.
- 3Department of Anatomy, College of Medicine, Dong-A University, Korea.
- 4Department of Oral and Maxillofacial Surgery, College of Dentistry, Pusan National University, Korea.
- 5Department of Biology, College of Natural Science, Dong-A University, Korea.
Abstract
- ABSTRACT: Apoptosis of osteosarcoma cells induced by bile duct derivates, HS-1200 was investigated with relation to mitochodria. HS-1200 induced cytochrome c and Smac/DIABLO release from mitochondria which are major factors related to apoptosis. In these apoptosis processes, release of cytochrome c was not blocked by caspase inhibitor, but release of Smac/DIABLO was blocked. BKA, a kind of PTP (permeablity transition pore) inhibitor, did not block both of them. Interestingly, the alteration of MMP was not observed by means of using JC-1 dye. Although MitoTracker, DiOC-6 and Rhodamine123 were used to confirm previous results, the decrease of MMP was not observed. In order to investigate whether this phenomenon is apoptosis-specific or cell-specific process, genistein was added to cells which usually decreased MMP. After adding genistein, MMP was not decreased, suggesting this phenomenon is cell-specific process. Conclusionally, HS-1200 induced apoptosis of osteosarcoma cells via mitochondria, cytochrome c and Smac/DIABLO were released from mitochondria without decrease of MMP. The release of Smac/DIABLO was dependent of caspase.
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