Korean J Physiol Pharmacol.  2006 Dec;10(6):337-341.

Alteration of Nitric Oxide Synthase and Guanylyl Cyclase Activity in Rats with Ischemia/Reperfusion Renal Injury

• Bae EH
• Kim SW

Abstract

The present study was designed to investigate the protein expression of nitric oxide synthase (NOS) and guanylyl cyclase (GC) activity in ischemia/perfusion (I/R) renal injury in rats. Renal I/R injury was experimentally induced by clamping the both renal pedicle for 40 min in Sprague-Dawley male rats. The renal expression of NOS isoforms was determined by Western blot analysis, and the activity of guanylyl cyclase was determined by the amount of guanosine 3', 5'-cyclic monophosphate (cGMP) formed in response to sodium nitroprusside (SNP), NO donor. I/R injury resulted in renal failure associated with decreased urine osmolality. The expression of inducible NOS (iNOS) was increased in I/R injury rats compared with controls, while endothelial NOS (eNOS) and neuronal NOS (nNOS) expression was decreased. The urinary excretion of NO metabolites was decreased in I/R injury rats. The cGMP production provoked by SNP was decreased in the papilla, but not in glomerulus. These results indicate an altered regulation of NOS expression and guanylyl cyclase activity in I/R-induced nephropathy.

Keyword

Nitric oxide synthase; Ischemic/perfusion injury; cGMP

MeSH Terms

Animals
Blotting, Western
Constriction
Guanosine
Guanylate Cyclase*
Humans
Male
Neurons
Nitric Oxide Synthase*
Nitric Oxide*
Nitroprusside
Osmolar Concentration
Protein Isoforms
Rats*
Rats, Sprague-Dawley
Renal Insufficiency
Tissue Donors
Guanosine
Guanylate Cyclase
Nitric Oxide
Nitric Oxide Synthase
Nitroprusside
Protein Isoforms