J Bacteriol Virol.
2002 Mar;32(1):39-45.
Identification of Fastidious Mycobacteria other than M. tuberculosis ( MOTT ) by Comparative Sequence Analysis of rpoB and 16S rDNA
- Affiliations
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- 1Department of Microbiology, Cheju National University College of Medicine, Cheju, Korea.
- 2Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
- 3The Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul, Korea.
- 4Department of Microbiology, Seoul National University College of Medicine, Korea. yhkook@plaza.snu.ac.kr
- 5Clinical Research Institute, SNUMRC, Seoul National University Hospital, Seoul, Korea.
- 6Institute of Endemic Diseases, Seoul National University College of Medicine, Korea.
Abstract
- Conventional tests for the identification of mycobacteria may frequently result in erroneous identification and underestimate the diversity within the genus Mycobacterium. However, this problem can be overcome by molecular approach like as 16S rRNA gene (rDNA) or RNA polymerase gene (rpoB) sequence analysis. In this study, a molecular approach analyzing partial sequence of 16S rDNA and rpoB gene was applied to mycobacteria other than M tuberculosis (MOTT) isolates that had not been definitely identified by conventional physical and biochemical tests. Among the eighteen isolates included in this study, twelve isolates could be identified to the species level and six were identified to the complex level. Compared with the results by 16S rDNA analysis, the rpoB analysis could di6erentiate some of the strains into the subspecies level.
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