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A Novel Mismatched PCR-Restriction Fragment Length Polymorphism Assay for Rapid Detection of gyrA and parC Mutations Associated With Fluoroquinolone Resistance in Acinetobacter baumannii

Kakuta N, Nakano R, Nakano A, Suzuki Y, Tanouchi A, Masui T, Horiuchi S, Endo S, Kakuta R, Ono Y, Yano H

BACKGROUND: Mutations in the quinolone resistance-determining regions (QRDRs) of Acinetobacter baumannii DNA gyrase (gyrA) and topoisomerase IV (parC) are linked to fluoroquinolone (FQ) resistance. We developed a mismatched PCR-restriction fragment...
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Development of Quantitative Real-Time PCR Primers for Detection of Streptococcus sobrinus

Park SN, Kook JK

The purpose of this study was to develop Streptococcus sobrinus-specific qPCR primers based on the nucleotide sequence of the RNA polymerase β-subunit gene (rpoB). The specificity of the primers was...
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In situ Hybridization for the Detection and Localization of the Bitter Taste Receptor Tas2r108 in the Murine Submandibular Gland

Ki SY, Cho YK, Chung KM, Kim KN

Mammals have 3 pairs of major salivary glands i.e., the parotid, submandibular, and sublingual glands. Saliva secretion of these glands is modulated by taste perception. Salivary glands are composed mainly...
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Detection of Helicobacter felis in a cat with gastric disease in laboratory animal facility

Hong S, Chung Y, Kang WG, Kim O

A 3-month-old male cat in the animal facility was presented for investigation of anorexia and occasional vomiting. We collected the specimens from gastroscopic biopsy and stool collection. The gastroscopic biopsy...
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A phosphorylation pattern-recognizing antibody specifically reacts to RNA polymerase II bound to exons

Han J, Lee JH, Park S, Yoon S, Yoon A, Hwang DB, Lee HK, Kim MS, Lee Y, Yang WJ, Youn HD, Kim H, Chung J

The C-terminal domain of RNA polymerase II is an unusual series of repeated residues appended to the C-terminus of the largest subunit and serves as a flexible binding scaffold for...
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Comparison of three diagnostic assays for the identification of Helicobacter spp. in laboratory dogs

Hong S, Chung Y, Kang WG, Choi YS, Kim O

A number of Helicobacter species may confound experimental data because of their association with disease progressing in various kinds of laboratory animals. Screening of Helicobacter species is particularly desirable, because...
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Development of Quantitative Real-Time PCR Primers for Detection of Prevotella intermedia

Park SN, Kook JK

Prevotella intermedia-specific quantitative real-time PCR (qPCR) primers were previously designed based on the nucleotide sequences of RNA polymerase beta-subunit gene (rpoB). However, the several clinical strains isolated from Korean populations...
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A New Record of Penicillium antarcticum from Marine Environments in Korea

Park MS, Lee EJ, Fong JJ, Sohn JH, Lim YW

  • KMID: 2312732
  • Mycobiology.
  • 2014 Jun;42(2):109-113.
During a survey of marine fungi from the waters surrounding Jeju Island, Korea, several Penicillium strains were isolated from seawater and marine sponges. Based on morphological characteristics and phylogenetic analyses...
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Understanding Rifampicin Resistance in Tuberculosis through a Computational Approach

Kumar S, Jena L

The disease tuberculosis, caused by Mycobacterium tuberculosis (MTB), remains a major cause of morbidity and mortality in developing countries. The evolution of drug-resistant tuberculosis causes a foremost threat to global...
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Molecular Discrimination of Mitis Group Streptococci Isolated from Koreans using RpoB Nucleotide Sequences

Park SN, Kook JK

  • KMID: 1431126
  • Int J Oral Biol.
  • 2013 Mar;38(1):29-36.
Mitis group streptococci (MGS) were classified based on the nucleotide sequences 16S rRNA gene (16S rDNA) and comprised 13 Streptococcus species. However, 16S rDNA homogeneity among MGS was too high...
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Tracing the Variation in Physiological Response to Rifampicin Across the Microbial Spectrum

Dey A, Chatterji

Rifampicin is an acknowledged inhibitor of bacterial RNA polymerase. We observed that there exists a substantial variation in the basal-level tolerance to rifampicin across microbial species. A number of mechanisms...
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Development of Quantitative Real-Time PCR Primers for the Detection of Aggregatibacter actinomycetemcomitans

Park SN, Park JY, Kook JK

  • KMID: 2135277
  • Int J Oral Biol.
  • 2011 Mar;36(1):1-6.
The purpose of this study was to develop species-specific real-time quantitative PCR (RT-qPCR) primers for use in the detection of Aggregatibacter actinomycetemcomitans. These primers were designed based on the nucleotide...
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New treatments for chronic hepatitis C

Jang JY, Chung RT

Treatments for chronic hepatitis C has evolved significantly in the past 15 years. The standard of care (SOC) is peginterferon alfa-2a/-2b with ribavirin for 48 weeks or 24 weeks in...
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Detection of Prevotella intermedia and Prevotella nigrescens using Pn17 and Pn34 DNA Probes

Park CH, Kim PS, Kim HS, Min JB, Hwang HK, Jang HS, Cho KW, Baek DH, Kook JK

  • KMID: 1719928
  • Int J Oral Biol.
  • 2010 Mar;35(1):13-19.
The DNA probes Pn17 and Pn34 were evaluated for their ability to specifically detect clinical strains of P. intermedia and P. nigrescens from a Korean population by dot blot hybridization....
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Diagnosis and Species Identification of Mycobacterial Infections by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Sterile Body Fluids

Cho CH, Han SH, Chin BS, Choi SH, Lee HS, Kim CO, Kim MS, Choi JY, Song YG, Kim JM

BACKGROUND/AIMS: The development of effective, accurate, and rapid diagnostic methods for Mycobacterium infection and mycobacterial species identification is required. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is an easy, rapid...
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Differentially Expressed Cellular Gene Profiles between Healthy HIV-infected Koreans and AIDS Patients

Choi JH, Choi BS, Kim SS, Lee JS

BACKGROUND: The global effect of HIV infection on the host cell gene expression profiles in healthy HIV-infected patients, as long-term non-progressors, remains largely unknown. To identify the cellular genes related...
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The Effect of TNF-alpha and INF-gamma on the Telomerase Activity of Cultured Human Keratinocyte

Hong KK, Lew BL, Kim YI, Lee JW, Kim NI

Psoriasis is a chronic inflammatory skin disease characterized by hyperproliferation and abnormal differentiation of the lesional epidermis. In pathogenesis, inflammatory cytokines such as TNF-alpha and IFN-gamma from infiltrated T-cells seem...
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Mutation of rpoB gene in M leprae isolates from Korea

Kim JP, Kim YS

  • KMID: 1512578
  • Korean Lepr Bull.
  • 2007 Dec;40(2):3-14.
Global efforts to control leprosy by intensive chemotherapy, including rifampin, have led to a significant decrease in the number of registered patients. But emergence of rifampin-resistant strains of pathogenic mycobacteria...
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Identification of Nontuberculous Mycobacteria by Sequence Analysis of the 16S Ribosomal RNA, the Heat-shock Protein 65 and the RNA Polymerase beta-Subunit Genes

Shin S, Kim EC, Yoon JH

BACKGROUND: The diagnosis of diseases caused by nontuberculous mycobacteria (NTM) is difficult, because NTM are prevalent in the environment such as soil and water, and because they have fastidious properties....
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Epidemiologic Characteristics and DNA Sequencing of SRSV Causing Gastroenteritis Outbreak in Two Elementary School

Ki HK, Jung YH, Jung JK, Seo JJ, Kim SH, Kee HY, Chun DS, Ji Y, Ha DR

  • KMID: 1808167
  • Infect Chemother.
  • 2003 Apr;35(2):107-114.
BACKGROUND: Small round structured virus (SRSV) is one of the common etiologic agents of viral gastroenteritis in childhood. However, SRSV has not easily been detected in many diarrheal outbreaks. Therefore,...
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