Korean J Urol.  1998 Mar;39(3):247-250.

Distinct Mutation of p53 Gene in Bladder Cancer of Smoking and Nonsmoking Patients

• Shim HY
• Heo YC
• Cho YH

Abstract

PURPOSE: It is well known that smoking is one of the most important etiologic factor in bladder cancer and mutations of p53 tumor suppressor gene are the important step in carcinogenesis of urinary bladder. In this study, we investigated the difference in pattern and rate of p53 gene mutation between smoker and non-smoker
MATERIALS AND METHODS
Of 26 bladder transitional cell carcinoma, 16 cases were smoker and 10 cases were non-smoker. We evaluated mutation of the p53 gene concentrated on axon 5 through 8, using polymerase chain reaction- single strand conformation polymorphism(PCR-SSCP) with radioisotope.
RESULTS
3 cases(18.7%) of 16 smoker were found to have p53 gene mutation, but none of 10 non-smoker was found. 2 of 3 cases of p53 gene mutation were found in exon 5 and 1 in exon 7. The pattern of p53 gene mutation was different in 3 cases.
CONCLUSIONS
Although the more cases will be needed in this study, we think that a mutation of p53 in bladder cancer may be associated with cigarette smoking.

Keyword

p53 mutation; Smoking; Transitional cell carcinoma; Bladder; PCR-SSCP

MeSH Terms

Axons
Carcinogenesis
Carcinoma, Transitional Cell
Exons
Genes, p53*
Genes, Tumor Suppressor
Humans
Smoke*
Smoking*
Urinary Bladder Neoplasms*
Urinary Bladder*
Smoke