Korean J Otolaryngol-Head Neck Surg.  1997 Sep;40(9):1299-1305.

Expression of p(53) Gene in Laryngeal Squamous Cell Carcinomas: Correlation with HPV Infection and Clinicopathological Findings

• Hwang CS
• Oh BK
• Yang HS
• Hong YH
• Kim H
• Kim CG
• Min HK

Abstract

BACKGROUND: Human papillomavirus(HPV) is epitheliotrophic virus invading the anogenital tract and the upper aerodigestive tract. HPV produces a diversity of benign and malignant tumors. The wild-type p(53) gene participates in suppressing cell transformation while the mutant forms have tumorigenic potential. Alterations in the structure of p(53) gene represent one of the most common genetic changes associated with human cancers.
OBJECTIVES
We evaluated the HPV infection and p(53) overexpression, and analyzed p(53) overexpression according to clinicopathological findings and HPV infection in laryngeal squamous cell carcinomas.
MATERIALS AND METHODS
Thirty-nine cases of laryngeal squamous cell carcinomas and ten cases of laryngeal nodules were analyzed for the detection of HPV DNA by in situ hybridization technique and the detection of p(53) overexpression by immunohistochemical technique.
RESULTS
1) HPV DNA was detected in 10(25.6%), and p(53) overexpression was detected in 19(48.7%) out of 39 cases in laryngeal squamous cell carcinomas. 2) The p(53) overexpression was detected in 7(78%) out of 9 cases more than Brinkman index score 1000, and was detected in 12(40%) out of 30 cases less than 1000. 3) HPV positive cases showed 50% of p(53) overexpression whereas HPV negative cases showed 48% overexpression.
CONCLUSION
HPV and p(53) gene were thought to be the etiological factors of laryngeal squamous cell carcinomas. The p(53) overexpression was related to smoking regardless of the histopathological findings and HPV infection.

Keyword

Human papillomavirus; p(53); Laryngeal carcinoma

MeSH Terms

Carcinoma, Squamous Cell*
DNA
Humans
In Situ Hybridization
Smoke
Smoking
DNA
Smoke