Korean J Gastroenterol.  1999 Nov;34(5):609-618.

Phenotypic Characteristics and CD2- and CD3-mediated Signal Transduction in HLA DR Positive and Negative Subsets of Lamina Propria-like T-cells

Abstract

BACKGROUND/AIMS: Peripheral blood T-cells co-cultured with Daudi (a irradiated B-cell line) and interleukin (IL)-2 attain a CD2 dominant cytokine secretion pattern and show tyrosine phosphorylation profile identical to freshly isolated lamina propria (LP) T-cells. Since the phenotypic characteristics and signal transduction pathway of these LP-like T-cells are largely unknown yet, we have explored the expression pattern of T-cell markers and CD2 ligation-induced tyrosine phosphorylation profile of LP-like T-cells and their HLA DR+ and DR- subsets.
METHODS
The LP-like T-cells were sorted into DR+ and DR- subsets by magnetic sorting. Surface markers were measured by flow cytometry. Tyrosine phosphorylation profile was investigated by Western blot.
RESULTS
The LP-like T-cells consisted of two distinct populations: approximately 55% of the cells express both HLA DR and IL-2R (DR+ LP-like T-cells) and the remainder express neither HLA DR nor IL-2R (DR- LP-like T-cells). Approximately 80% of DR+ LP-like T-cells were memory cells expressing CD45RO and 2/3 of them were activated T-cells expressing CD69, CD71 and CD30. In contrast, 50% of DR- LP-like T-cells expressed CD45RO and only 10-20% of them expressed activation markers. Tyrosine phosphorylation of p72 protein after CD2 ligation was identified only in DR- LP-like T-cells.
CONCLUSIONS
The LP-like T-cells consist of two distinct subpopulation in terms of phenotype and tyrosine phosphorylation pattern according to CD2 ligation.

Keyword

Lamina propria T-cells; LP-like T-cells; Phenotype; CD2; Tyrosine phosphorylation

MeSH Terms

B-Lymphocytes
Blotting, Western
Flow Cytometry
Interleukins
Ligation
Memory
Mucous Membrane
Phenotype
Phosphorylation
Signal Transduction*
T-Lymphocytes*
Tyrosine
Interleukins
Tyrosine
Full Text Links
  • KJG
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
    DB Error: unknown error