J Korean Soc Microbiol.  1999 Oct;34(5):423-433.

Characterization of a Vibrio parahaemolyticus Phage Isolated from Marine

Abstract

A novel bacteriophage, designated as VPP97, that infects the strains of Vibiro parahaemolyticus (hallophilic, Gram-negative bacterium) isolated most commonly from marine environments, has been discovered, and several of its properties have been determined. The plaques were clear and sized 0.6-1.0 mm in diameter. The virion forms a single band on 70% sucrose gradient and p1.50 CsC1 gradient by sucrose gradient centrifugation and CsCI gradient centrifugation respectively. It has a hexagonal head and a relatively long tail, as shown by electron microscopy. Vibrio alginolyticus, Vibrio fluvialis and Vibrio furnissii were also sensitive to this phage It was almost totally inactivated at 70 degree C and at pH below 5 or over 10. The nucleic acid of VPP97 is composed of DNA. The VPP97 had 9 specific structural proteins sized between 21.5 kDa and 97.4 kDa on SDS-PAGE. When V. parahaemolyticus cultures were treated with either phage VPP97 or one of the several antibiotics for 2 hours, the viable number of V. parahaemolyticus treated with the phage VPP97 is lower than that treated with chloramphenicol, erythromycin or penicillin, but not lower than that treated with tetracycline. Mice that have responded to the phage treatment revealed the lower numbers of V. parahaemolyticus in small intestine and less damage on small intestine compared to the untreated mice. Therefore, we suggest that the phage treatment appears effective to the infection by V. parahaemolyticus.

Keyword

Vibrio parahaemolyticus phages; Characterization of phages; Phage therapy

MeSH Terms

Animals
Anti-Bacterial Agents
Bacteriophages*
Centrifugation
Chloramphenicol
DNA
Electrophoresis, Polyacrylamide Gel
Erythromycin
Head
Hydrogen-Ion Concentration
Intestine, Small
Mice
Microscopy, Electron
Penicillins
Sucrose
Tail
Tetracycline
Vibrio alginolyticus
Vibrio parahaemolyticus*
Vibrio*
Virion
Anti-Bacterial Agents
Chloramphenicol
DNA
Erythromycin
Penicillins
Sucrose
Tetracycline
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