J Korean Cancer Assoc.  1997 Feb;29(1):29-37.

Anti-tumor Effect of the Complex of Acriflavine and Guanosine (AG60)

• Hong EK
• Kim HM
• Lee KY
• Chung YS
• Yoo BI
• Kim SG
• Ahn ET
• Han YB

Abstract

PURPOSE: The anti-tumor effect of the complex of acriflavine and guanosine (AG60) was investigated.
MATERIALS AND METHODS
In vitro cytotoxicity of AG60 was measured using SRB assay, and in vivo antitumor activity of AG60 was examined in CDF1 mice intraperitoneally inoculated with the P388 leukemic cells and in ICR mice inguinally implanted with S-180 cells. Tumor size and mean survival time were determined.
RESULTS
AG60 and acriflavine showed strong anti-tumor effect in vitro on lung cancer (A549), renal cancer (UO-31) and colon cancer (COLO205) cells. However, AG60 did not show the cytotoxicity against normal cell line, 3T3. The range of the IC50 of AG60 to the various tumor cell lines was 0.09 microgram/ml through 1.94 microgram/ml. The treatment of ascitic tumor bearing CDF1 mice with AG60 resulted in over 160% increases in the mean survival time. The most effective dose of AG60 was 30 mg/kg body weight in tumor implanted mice. In solid tumor bearing ICR mice tumor growth and progression were suppressed in response to the different doses at 30 days; 69.8% suppression of tumor size in response to acriflavine, 16.0% to guanosine, 87.7% to AG60 and 78.5% to doxorubicin. In addition, 35% increases were observed in the means survival time of AG60 treated group compared with control group.
CONCLUSION
The prominant anti-tumor effects of AG60 shown in this report would represent the possibility of the clinical trials.

Keyword

Acriflavine; Guanosine; Cytotoxicity; Tumor growth inhibition

MeSH Terms

Acriflavine*
Animals
Body Weight
Cell Line
Cell Line, Tumor
Colonic Neoplasms
Doxorubicin
Guanosine*
Inhibitory Concentration 50
Kidney Neoplasms
Lung Neoplasms
Mice
Mice, Inbred ICR
Survival Rate
Acriflavine
Doxorubicin
Guanosine