Korean J Fertil Steril.  1997 Aug;24(2):253-259.

Chromosome Configurations of Human Oocytes Matured in vitro following Cryopreservation at the Germinal Vesicle Stage

• Park SE
• Chung CJ
• Son WY
• Chung HM
• Lee SH
• Lee WS
• Ko JJ
• Yoon TK
• Cha KY

Abstract

OBJECTIVE
To investigate effects of cryoprotectant and cryopreservation on the chromosome of the human immature oocytes. DESIGN: Intact cumulus-enclosed immature oocytes were collected from unstimulated ovaries and divided into three groups, such as no treatment as control (group 1), only 1,2-propanediol (PROH)-treated (group 2), and cryopreserved oocytes (group 3). Oocytes in group 1, 2, and survived oocytes after cryopreservation in group 3 were cultured for 48 hours. SETTING: Infertility Medical Center at the CHA General Hospital, Seoul, Korea. PATIENTS: Oocytes were obtained from Patients undergoing gynecological surgery. MAIN OUTCOME MEASURES: Maturation rate, abnormality in chromosomes by fluorescence in situ hybridization (FISH).
RESULTS
There was no effect of PROH only treatment on the chromosomal abnormalities in group 2 compared to control oocytes (41.4% and 31.8%, respectively). Whereas significantly increased abnormalities in chromosome (77.8%) were found in group 3.
CONCLUSIONS
Human oocytes matured in vitro after cryopreservation at the germinal vesicle (GV) stage showed increased incidence of chromosomal abnormalities. These abnormalities may impair the capacity for further development of the embryos derived from frozen-thawed oocytes.

MeSH Terms

Chromosome Aberrations
Cryopreservation*
Embryonic Structures
Female
Fluorescence
Gynecologic Surgical Procedures
Hospitals, General
Humans*
In Situ Hybridization
Incidence
Infertility
Korea
Oocytes*
Outcome Assessment (Health Care)
Ovary
Propylene Glycol
Seoul
Propylene Glycol