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A survey on the awareness and knowledge about elective oocyte cryopreservation among unmarried women of reproductive age visiting a private fertility center

Hong YH, Park JW, Kim H, Kim SK, Choo CW, Jee BC, Suh CS, Kim SH

OBJECTIVE: The aim of this study was to investigate the level of awareness and knowledge regarding elective oocyte cryopreservation (OC) among unmarried women of reproductive age in Korea. METHODS: A survey...
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The Effect of Cryopreservation of Allograft Nerve on the Recovery of Motor Function

Choi SS, Park I, Hyun JK, Jung JU, Kim JP

PURPOSE: Peripheral nerve allograft can be an acceptable alternative, but it has not yet become clinically useful because of immune response to foreign tissue. With significant advances in the research...
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Long-Term Experience of Sperm Cryopreservation in Cancer Patients in a Single Fertility Center

Song SH, Kim DK, Sung SY, Her YS, Lee OH, Choi MH, Kim HK, Lyu SW, Kim DS

PURPOSE: Sperm cryopreservation before cancer treatment is the most effective method to preserve the fertility of male patients. We present our 21 years experience with sperm cryopreservation for cancer patients,...
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Iodixanol supplementation during sperm cryopreservation improves protamine level and reduces reactive oxygen species of canine sperm

Abdillah D, Setyawan E, Oh HJ, Ra K, Lee SH, Kim MJ, Lee BC

The objective of this study was to analyze the protective effects of iodixanol on dog spermatozoa during cryopreservation. The optimal concentration of iodixanol, 1.5%, was determined using fresh spermatozoa and...
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Angiopoietin-1 and -2 and vascular endothelial growth factor expression in ovarian grafts after cryopreservation using two methods

Cho IA, Lee YJ, Lee HJ, Choi IY, Shin JK, Lee SA, Lee JH, Choi WJ

OBJECTIVE: The favored method of preserving fertility in young female cancer survivors is cryopreservation and autotransplantation of ovarian tissue. Reducing hypoxia until angiogenesis takes place is essential for the survival...
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Optimization of Large-Scale Expansion and Cryopreservation of Human Natural Killer Cells for Anti-Tumor Therapy

Min B, Choi H, Her JH, Jung MY, Kim HJ, Jung MY, Lee EK, Cho SY, Hwang YK, Shin EC

Allogeneic natural killer (NK) cell therapy is a potential therapeutic approach for a variety of solid tumors. We established an expansion method for large-scale production of highly purified and functionally...
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Ovarian tissue cryopreservation and transplantation in patients with cancer

Kim S, Lee Y, Lee S, Kim T

Chemotherapy and radiotherapy improved survival rates of patients with cancer. However, they can cause ovarian failure and infertility in women of reproductive age. Infertility following cancer treatment is considered a...
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Cryopreservation of Cartilage for Transplantation in Rhinoplasty: A Literature Review

Mutusamy S, Na WS, Jin HR

Although many rhinosurgeons still use cryopreserved septal or rib cartilage for grafts in rhinoplasty, there is no consensus whether it is an effective method preserving the chondrocyte viability. To propose...
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Cryopreservation of in vitro matured oocytes after ex vivo oocyte retrieval from gynecologic cancer patients undergoing radical surgery

Park CW, Lee SH, Yang KM, Lee IH, Lim KT, Lee KH, Kim TJ

OBJECTIVE: The aim of this study was to report a case series of in vitro matured (IVM) oocyte freezing in gynecologic cancer patients undergoing radical surgery under time constraints as...
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Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar

Kim JW, Kim TY, Kim YM, Pang EK, Kim SJ

PURPOSE: This study was conducted to determine cell viability and differentiation capability of human periodontal ligament (PDL) cells and to elucidate the effects of cryopreservation on the activity of human...
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A System for Cryopreservation of Rare Red Blood Cell Units; Right Time to Start

Cho EH, Chun S, Seo JY, Kang ES, Cho D, Kim DW

No abstract available.
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Cryopreservation of Human Wharton's Jelly-derived Mesenchymal Stem Cells Following Controlled Rate Freezing Protocol Using Different Cryoprotectants; A Comparative Study

Shivakumar SB, Bharti D, Jang SJ, Hwang SC, Park JK, Shin JK, Byun JH, Park BW, Rho GJ

OBJECTIVES: To compare the effect of three different cryoprotectants on basic stem cell characteristics for the possibility of using well defined, dimethyl sulfoxide (DMSO) and serum free freezing solutions to...
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Semen Analysis in Cancer Patients Referred for Sperm Cryopreservation before Chemotherapy over a 15-Year Period in Korea

Ku JY, Park NC, Jeon TG, Park HJ

PURPOSE: This study evaluated the demographics and semen parameters of males with cancer who banked their sperm prior to chemotherapy. MATERIALS AND METHODS: This is a retrospective study of 66 cases...
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Cryopreservation of dental tissue and subsequent isolation of mesenchymal stem cells

Park BW

No abstract available.
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Vitrification solution without sucrose for cryopreservation in mouse blastocysts

Joo JK, Lee YJ, Jeong JE, Kim SC, Ko GR, Lee KS

OBJECTIVE: This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution. METHODS: Mouse two-cell embryos were collected...
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Effects of Long-Term Cryopreservation on Fat Grafts: An Experimental Study

Lee HJ, Kim EK

BACKGROUND: Although microfat grafting is now used to augment soft tissue, resorption of some amount of fat is inevitable. There are no consistent guidelines for the duration of fat storage....
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Comparison of Vitrification and Slow Freezing for the Cryopreservation of Mouse Pronuclear Stage Embryos

Kim MY, Lee YI

  • KMID: 2288278
  • Korean J Reprod Med.
  • 2007 Jun;34(2):117-124.
OBJECTIVE: The aim of this study was to compare the efficacy of slow freezing with vitrification method for cryopreservation of mouse pronuclear stage embryos. METHODS: Mouse pronuclear embryos obtained from superovulated...
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Ovarian tissue cryopreservation and transplantation

Park KH, Lee BS, Chung DJ

  • KMID: 2272986
  • Korean J Obstet Gynecol.
  • 2006 Dec;49(12):2473-2478.
This review focuses on the current options for fertility preservation in patients with high risk of premature ovarian failure. Available cryopreservation options include embryo cryopreservation, oocyte cryopreservation, and ovarian tissue...
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Experimental Study on Cryopreservation of Pancreas Islet Cell in Rat

Back JH, Kim SC, Kim IH, We YM, Kim YH, Kim JH, Cho IH, Cho EY, Lim DG, Han DJ

  • KMID: 2201805
  • J Korean Soc Transplant.
  • 2005 Dec;19(2):124-130.
PURPOSE: Cryopreservation of pancreas islet cells can facilitate the clinical islet transplantation by giving a means of storage of islets and immunomodulation on pancreatic islet preparations. METHODS: Pancreatic islets were isolated...
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Cryopreservation and Thawing of Red Blood Cells Using Haemonetics ACP 215

Jung OJ, Kim MJ, Lee MK, Chung HR, Oh DJ, Lim AH, Han TH

  • KMID: 2238964
  • Korean J Lab Med.
  • 2005 Oct;25(5):347-351.
BACKGROUND: The FDA has approved the storage of frozen red blood cells (RBCs) at -80degrees C for 10 years. After deglycerolization, the RBCs can be stored at 4degrees C for...
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