J Korean Androl Soc.
1995 Dec;13(2):77-80.
Estimation of Damage to Human Spermatozoa During Cryopreservation by Measurement of Release of Glutamic-Oxalacetic Transaminase
- Affiliations
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- 1Department of Urology, College of Medicine, Yonsei University, Seoul, Korea.
Abstract
- Release of glutamic-oxalacetic transaminase (GOT) has been used as an indicator of damage during cryopreservation of spermatozoa from domestic animals. This study was planned to determine whether changes in seminal GOT activity could be measured after cryopreservation of human spermatozoa and to correlate the findings with the extent of damage detected with eosinstaining. Seminal GOT correlated positively with sperm density, and increase were measured after cryopreservation. The amount of GOT released was not influenced by the speed of cryopreservation (fast vs. slow) and reflected the extent of damage to the plasma membrane of the spermatozoa seen by light microscopy. Measurement of GOT may be a useful indicator of the cellular integrity of cryopreserved spermatozoa.
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