Tuberc Respir Dis.  1968 Sep;15(3):5-7. 10.4046/trd.1968.15.3.5.

Studies on Mycobacterial Transaminases

• KMID: 2529177
• DOI: http://doi.org/10.4046/trd.1968.15.3.5

Abstract

lt is included on present papers that the enzyme activities of Glutamic oxaloacetic(GOT) and Glutamic Pyruvic Transaminase(GPT) were testified with several strains of human tubercle bacilli and other Mycobacteria (Scotochromogen and M. Smegmatis.). Calculation of both enzyme activities were conducted with Wroblewski’s method (GPT) and Umbreit's method (GOT) in both techniques the formation of oxaloacetic or Pyruvic acid frorn L-ketoglutaric acid and aspartic or alanine are catalyzed by the Mycobacterial GOT or GPT The Oxaloacetic or Pyruvic acid forrned are then reacted with dinitrophenylhydrazine to forrn the colored hydrazones which are measured spectrophotometrically. Glutamic Oxaloacetic Transaminase activities of the tested' strains are following as: Drug sensitive tubercle bacilli isolated from TB patients, 42, 47 units; INH resistant human tubercle bacilli, 40, 41 units; H₃₇Rv 53units; Scotochromogen(one strain), units; M. smegmatis, 12 units. Glutamic Pyruvic Transaminase activities of the tested strains are following as: Drug sensitive tubercle bacilli, 14, 18 units; INH resistant tubercle bacilli, 32, 33 units; H₃₇Rv, 20 units; Scotochromogen, 44units; M. Smegmatis, 7units.