Korean J Parasitol.
1992 Jun;30(2):151-153. 10.3347/kjp.1992.30.2.151.
The maintenance of free-living amoebae by cryopreservation
- Affiliations
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- 1Department of Parasitology, College of Medicine, Yonsei University, Seoul, Korea.
- KMID: 1044818
- DOI: http://doi.org/10.3347/kjp.1992.30.2.151
Abstract
- We have successfully cryopreserved free-living amoebae in order to maintain them feasibly under the conditions in our laboratory. The viability of trophozoites was higher when frozen by slow cooling (overall 0.7 degree C/min) than by fast cooling (overall 1.3 degrees C/min). Glycerol and dimethylsulfoxide at the final concentration of 7.5% each was used for cryopreservation of free-living amoebae trophozoites. The survival rate was 2-39% after storage in the liquid nitrogen for 60 days. Gross cultural or morphological changes were not noted in trophozoites thawed from frozen suspensions.
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