Korean J Physiol Pharmacol.  2007 Aug;11(4):149-154.

Identification of Differentially Expressed Genes in Murine Hippocampus by Modulation of Nitric Oxide in Kainic Acid-induced Neurotoxic Animal Model

• Suh YA
• Kwon OM
• Yim SY
• Lee HJ
• Kim SS

Abstract

Kainic acid (KA) causes neurodegeneration, but no consensus has been reached concerning its mechanism. Nitric oxide may be a regulator of the mechanism. We identified differentially expressed genes in the hippocampus of mice treated with kainic acid, together with or without L-NAME, a nonselective nitric oxide synthase inhibitor, using a new differential display PCR method based on annealing control primers. Eight genes were identified, including clathrin light polypeptide, TATA element modulatory factor 1, neurexin III, ND4, ATPase, H+ transporting, V1 subunit E isoform 1, and N-myc downstream regulated gene 2. Although the functions of these genes and their products remain to be determined, their identification provides insight into the molecular mechanism(s) involved in KA-induced neuronal cell death in the hippocampal CA3 area.

Keyword

Excitotoxicity; Kainic acid; L-NAME; Differential display PCR; Hippocampus

MeSH Terms

Animals*
Cell Death
Clathrin
Consensus
Hippocampus*
Kainic Acid
Mice
Models, Animal*
Neurons
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Nitric Oxide*
Polymerase Chain Reaction
Proton-Translocating ATPases
Clathrin
Kainic Acid
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase
Proton-Translocating ATPases