J Korean Acad Periodontol.  2008 Aug;38(Suppl):299-308. 10.5051/jkape.2008.38.Suppl.299.

DNA microarray analysis of gene expression of MC3T3-E1 osteoblast cell cultured on anodized- or machined titanium surface

• Park JM
• Jeon HR
• Pang EK
• Kim MR
• Kang NR

• KMID: 1486864
• DOI: http://doi.org/10.5051/jkape.2008.38.Suppl.299

Abstract

PURPOSE
The aim of this study was to evaluate adhesion and gene expression of the MC3T3-E1 cells cultured on machined titanium surface (MS) and anodized titanium surface (AS) using MTT test, Scanning electron micrograph and cDNA microarray.
MATERIALS AND METHODS
The MTT test assay was used for examining the proliferation of MC3T3-E1 cells, osteoblast like cells from Rat calvaria, on MS and AS for 24 hours and 48 hours. Cell cultures were incubated for 24 hours to evaluate the influence of the substrate geometry on both surfaces using a Scanning Electron Micrograph (SEM). The cDNA microarray Agilent Rat 22K chip was used to monitor expressions of genes.
RESULTS
After 24 hours of adhesion, the cell density on AS was higher than MS (p<0.05). After 48 hours the cell density on both titanium surfaces were similar (p>0.05). AS had the irregular, rough and porous surface texture. After 48 hours incubation of the MC3T3-E1 cells, connective tissue growth factor (CTGF) was up-regulated on AS than MS (more than 2 fold) and the insulin-like growth factor 1 receptor was down-regulated (more than 2 fold) on AS than MS.
CONCLUSION
Microarray assay at 48 hours after culturing the cells on both surfaces revealed that osteoinductive molecules appeared more prominent on AS, whereas the adhesion molecules on the biomaterial were higher on MS than AS, which will affect the phenotype of the plated cells depending on the surface morphology.

Keyword

cDNA microarray; MTT assay; SEM; anodized titanium surface

MeSH Terms

Animals
Cell Count
Cell Culture Techniques
Connective Tissue Cells
DNA
DNA, Complementary
Electrons
Gene Expression
Oligonucleotide Array Sequence Analysis
Organothiophosphorus Compounds
Osteoblasts
Phenotype
Rats
Skull
Titanium
DNA
DNA, Complementary
Organothiophosphorus Compounds
Titanium