Infect Chemother.  2008 Feb;40(1):32-39. 10.3947/ic.2008.40.1.32.

Distribution of Genes Encoding Aminoglycoside Modifying Enzymes and Type Staphylococcal Chromosomal Cassette mec in Methicillin-resistant Staphylococcus aureus from Non-tertiary Hospitals

• Jung YH
• Kim KW
• Cha JO
• Lee KM
• Yoo JI
• Yoo JS
• Kim BS
• Oh YJ
• Yoon HR
• Lee YS

• KMID: 1483474
• DOI: http://doi.org/10.3947/ic.2008.40.1.32

Abstract

BACKGROUND: Many genes encoding aminoglycoside modifying enzymes (AMEs) on transposon or plasmid were transferred from one strain to another strain and inserted into a staphylococcal chromosomal cassette mec (SCCmec). There are very diverse subtypes in SCCmec type to the insertion of resistant genes. Therefore, we researched the resistance rates of antibiotics and distribution of AME genes according to SCCmec type in MRSA strains.
MATERIALS AND METHODS
We isolated 640 Staphylococcus aureus from non-tertiary hospitals in 2004, detected mecA, aac(6')-aph(2"), aph(3')-IIIa, and ant(4')-Ia using the multiplex PCR method, tested antibacterial susceptibility disk diffusion and minimal inhibitory concentration, and determined SCCmec type.
RESULTS
Of 640 S. aureus isolates, MRSA rate was 39.7% and all MRSA isolates carried mecA gene. Among 214 MRSA selected, aminoglycoside-resistant rates were 98.1% in kanamycin and tobramycin, 68.7% in gentamicin, 30.8% in amikacin, and 2.8% in netilmicin. The detection rates for aac(6')-aph(2"), aph(3')-IIIa, and ant(4')-Ia were 77.1%, 13.1%, and 53.3%, respectively. Also, SCCmec type was 50.9% in SCCmec type II, 16.4% in type III, and 32.7% in type IV. The genes encoding AMEs were distributed aac(6')-aph(2") (49.5%) and aac(6')-aph(2")/ant(4')-Ia (36.7%) in SCCmec type II, aph(3')-IIIa/aac(6')-aph(2") (60%) and aac(6')-aph(2") (31.4%) in type III, and aac(6')-aph(2")/ant(4')-Ia (41.4%) and ant(4')-Ia (50%) in type IV.
CONCLUSION
39.7% of S. aureus isolated from non-tertiary hospitals was resistant to methicillin. More than 90% of MRSA isolates were detected aac(6')-aph(2") in SCCmec type II and III, and ant(4')-Ia in type IV. With these results, the genes encoding AMEs may be closed related to SCCmec type.

Keyword

Aminoglycoside modifying enzyme; SCCmec; Methicillin resistant; Staphylococcus aureus

MeSH Terms

Adenosine
Amikacin
Amphotericin B
Anti-Bacterial Agents
Diffusion
Gentamicins
Kanamycin
Kanamycin Kinase
Methicillin
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Multiplex Polymerase Chain Reaction
Netilmicin
Plasmids
Sprains and Strains
Staphylococcus aureus
Tobramycin
Adenosine
Amikacin
Amphotericin B
Anti-Bacterial Agents
Gentamicins
Kanamycin
Kanamycin Kinase
Methicillin
Netilmicin
Tobramycin

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