Korean J Clin Microbiol.  2008 Oct;11(2):98-106. 10.5145/KJCM.2008.11.2.98.

Characteristics of Acquired beta-lactamase Gene in Clinical Isolates of Multidrug-resistant Pseudomonas aeruginosa

• Chung SY
• Sung JY
• Kwon KC
• Park JW
• Ko CS
• Shin SY
• Song JH
• Koo SH

• KMID: 1480976
• DOI: http://doi.org/10.5145/KJCM.2008.11.2.98

Abstract

BACKGROUND: Recently, there have been reports of infections with multidrug-resistant Pseudomonas aeruginosa. To determine the mechanism of the resistance, we investigated the prevalence of Ambler class A and D beta-lactamases, their extended-spectrum derivatives, and class B and D carbapenemase in multidrug-resistant P. aeruginosa isolates.
METHODS
During the period of March 2006 to May 2007, clinical isolates of multidrug-resistant P. aeruginosa were collected from patients in Chungnam National University Hospital, Daejeon, Korea. Inhibitor-potentiated disk diffusion tests were used for the screening of metallo-beta-lactamase (MBL) production. PCR and DNA sequencing were conducted for the detection of beta-lactamase genes. We also employed the enterobacterial repetitive intergenic consensus (ERIC)- PCR method for an epidemiologic study.
RESULTS
A total of 37 consecutive, non-duplicate, multidrug-resistant P. aeruginosa were isolated. Twenty- nine of 37 isolates harbored blaOXA-10 (56.8%), blaOXA-2 (18.9%), and blaOXA-1 (5.4%). Only one isolate produced IMP-1, and it also harbored blaOXA-1. None harbored Ambler class A beta-lactamase or class D carbapenemase. The strains producing OXA type beta-lactamases showed a significantly higher resistance to aminoglycoside compared to non-producers. The ERIC-PCR pattern of the 19 OXA-10 producing strains indicated that the isolates were closely related in terms of clonality.
CONCLUSION
OXA type beta-lactamases are the most prevalent among the acquired beta-lactamases produced by multidrug-resistant P. aeruginosa isolated at a university hospital in Chungcheong Province. Besides beta-lactam antibiotics, the strains harboring OXA type beta-lactamase showed a significantly higher resistance to aminoglycoside and qunolone.

Keyword

Multidrug resistance; Pseudomonas aeruginosa; OXA type beta-lactamase; Metallo-beta- lactamase (MBL)

MeSH Terms

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactamases
Consensus
Diffusion
Drug Resistance, Multiple
Epidemiologic Studies
Humans
Korea
Mass Screening
Oxytocin
Polymerase Chain Reaction
Prevalence
Pseudomonas
Pseudomonas aeruginosa
Sequence Analysis, DNA
Anti-Bacterial Agents
Bacterial Proteins
Oxytocin
beta-Lactamases

Figure

• 

  Fig. 1. ERIC-PCR patterns of genomic DNA from clinical isolates of multi-drug resistant Pseudomonas aeruginosa harboring blaOXA-10. Lane M is 1 kb DNA size marker. Nineteen strains of OXA-10 β-lactamase producing clinical isolates show A or B pattern.

Reference

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