Korean J Leg Med.  2009 Nov;33(2):153-158.

Species Specificity of a Commercial AmpFLSTR(R) MiniFiler(TM) PCR Amplification Kit

Affiliations
  • 1Department of Forensic Medicine Seoul National University College of Medicine, Seoul, Korea. sdlee@snu.ac.kr
  • 2Institute of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea

Abstract

Recently forensic scientists have focused on extending the applicability of STR to degraded or tiny amount DNA. Short amplicon may be one of the solutions to these samples and several commercial kits are available for this purpose. Before practical casework, validation is necessary for newly introduced kit. We tried to check how newly introduced STR kit, AmpFLSTR(R) MiniFiler(TM) PCR Amplification Kit, reacts with some animal DNAs. We tried 27 animal DNA samples and checked whether the above kit amplify animal DNA, and next how the band appears on electropherogram. We compared the results with popular STR kits. With AmpFLSTR(R) MiniFiler(TM) PCR Amplification Kit, we could get several bands on electropherogram for some species, but we could not designate proper allelic number compared to allelic ladder for most loci except the following loci, D13S317, D2S1338, D21S11, D18S51, FGA. The D18S51 locus was outstanding in that some species showed definite designated alleles, but the allelic number was not different depending on species. This was the same with another popular STR kit, AmpFLSTR(R) Identifiler(TM) PCR Amplification Kit, which was from the same company. The another kit from another company, PowerPlex(R) 16 System showed different phenomenon with more increased number of amplified bands which were usually differ on size when compared to allelic ladder.

Keyword

Species Specificity; STR; Forensic; MiniFiler

MeSH Terms

Alleles
Animals
DNA
Polymerase Chain Reaction
Species Specificity
DNA
Full Text Links
  • KJLM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr