Korean J Leg Med.  2009 May;33(1):79-83.

Efficiency of Quantification using QuantifilerTM Human DNA Kit in 60 Year Old Bone

• Zhang A
• Seo SB
• Yi J
• Lee HY
• Kim HY
• Shin DH
• Kang HW
• Lee SD

Abstract

Commercial kits usually recommend certain range of the amount of DNA without PCR inhibitors for optimal results. If proper DNA is not used in STR amplification, undesirable results such as enhanced stutter effects, split peaks, allele dropout and poor peak balance could be shown. This phenomenon would happen more frequently in case of bone typing. Therefore, we checked effect of DNA concentration and PCR inhibitors on conventional STR typing results using QuantifilerTM Human DNA Quantification Kit. The 54 bone samples over a 60-year burial period were used in this study. In all samples, the DNA concentration ranged from 0.8 pg/ul to 748 pg/ul and Ct values of IPC that indicates residue of PCR inhibitors ranged from 26.69 to 35.59. The DNA concentration and the number of amplified loci showed positive correlation. The number of loci amplified using Minifiler kit was much more than using Y filer kit. Samples with high Ct value of IPC were not almost amplified with Y filer kit. These results suggested that QuantifilerTM Kit is useful to obtain information for DNA typing.

Keyword

forensic science; quantification; bone, inhibition; commercial kit

MeSH Terms

Alleles
Burial
DNA
DNA Fingerprinting
Forensic Sciences
Humans
Patient Dropouts
Polymerase Chain Reaction
DNA