Gut Liver.  2009 Jun;3(2):88-94. 10.5009/gnl.2009.3.2.88.

The Clinicopathological Significance of Tissue Levels of Hypoxia-Inducible Factor-1alpha and Vascular Endothelial Growth Factor in Gastric Cancer

• Kim SE
• Shim KN
• Jung SA
• Yoo K
• Lee JH

• KMID: 1464750
• DOI: http://doi.org/10.5009/gnl.2009.3.2.88

Abstract

BACKGROUND/AIMS
Hypoxia-inducible factor-1alpha (HIF-1alpha) is a mediator of tumor progression. Vascular endothelial growth factor (VEGF) is the most potent angiogenic factor known to be induced by HIF-1alpha. We investigated the clinicopathological significance of HIF-1alpha and VEGF levels in biopsied gastric cancer tissue.
METHODS
Endoscopic biopsy specimens from 67 patients with gastric carcinoma who underwent surgery were available for this study. Semiquantitative RT-PCR was applied to biopsied tumors and normal tissues to determine the expressions of HIF-1alpha and VEGF. The expression levels of HIF-1alpha and VEGF were evaluated using the tumor:normal (T/N) ratios of HIF-1alpha and VEGF mRNA. The clinicopathological variables were reviewed retrospectively.
RESULTS
The T/N ratios of HIF-1alpha mRNA showed significant correlation with lymph-node metastases, distant metastases, stage, and recurrence within 3 years (p<0.05). The T/N ratios of VEGF mRNA showed significant correlation with lymph-node metastases and distant metastases (p<0.05). There was a significant correlation between the T/N ratios of HIF-1alpha and VEGF mRNA (r=0.72, p<0.01).
CONCLUSIONS
The increased expression of HIF-1alpha and VEGF mRNA could reflect aggressive tumor behavior, including the recurrence of gastric cancer. Examination of HIF-1alpha mRNA in biopsy specimens by RT-PCR assay might provide useful preoperative information on tumor aggressiveness.

Keyword

Stomach neoplasms; Hypoxia-inducible factor 1, alpha; Vascular endothelial growth factor

MeSH Terms

Angiogenesis Inducing Agents
Biopsy
Humans
Neoplasm Metastasis
Recurrence
RNA, Messenger
Stomach Neoplasms
Vascular Endothelial Growth Factor A
Angiogenesis Inducing Agents
RNA, Messenger
Vascular Endothelial Growth Factor A