Exp Neurobiol.  2010 Sep;19(2):75-82. 10.5607/en.2010.19.2.75.

A Winged-Helix Transcription Factor Foxg1 Induces Expression of Mss4 Gene in Rat Hippocampal Progenitor Cells

• Nam JS
• Yang H
• Kim NH
• Sun Y
• Choi BS
• Huh SO

• KMID: 1460703
• DOI: http://doi.org/10.5607/en.2010.19.2.75

Abstract

Foxg1 (previously named BF1) is a winged-helix transcription factor with restricted expression pattern in the telencephalic neuroepithelium of the neural tube and in the anterior half of the developing optic vesicle. Previous studies have shown that the targeted disruption of the Foxg1 gene leads to hypoplasia of the cerebral hemispheres with severe defect in the structures of the ventral telencephalon. To further investigate the molecular mechanisms by which Foxg1 plays essential roles during brain development, we have adopted a strategy to isolate genes whose expression changes immediately after introduction of Foxg1 in cultured neural precursor cell line, HiB5. Here, we report that seventeen genes were isolated by ordered differential displays that are up-regulated by over-expression of Foxg1, in cultured neuronal precursor cells. By nucleotide sequence comparison to known genes in the GeneBank database, we find that nine of these clones represent novel genes whose DNA sequences have not been reported. The results suggest that these genes are closely related to developmental regulation of Foxg1.

Keyword

Foxg1; telencephalon development; Mss4; ordered-differential display

MeSH Terms

Animals
Base Sequence
Brain
Cell Line
Cerebrum
Clone Cells
Neural Tube
Neurons
Rats
Stem Cells
Telencephalon
Transcription Factors
Transcription Factors