Clin Exp Otorhinolaryngol.  2010 Jun;3(2):84-90. 10.3342/ceo.2010.3.2.84.

Transplantation of Neural Stem Cells in Anosmic Mice

• Lee CH
• Jeon SW
• Seo BS
• Mo JH
• Jeon EH
• Choi AR
• Kim JW

• KMID: 1460594
• DOI: http://doi.org/10.3342/ceo.2010.3.2.84

Abstract

OBJECTIVES
Treating olfactory dysfunction is a challenge for physicians. One of the therapeutic options could be transplantation of stem cells. In this study, neural stem cells were transplanted into anosmic mice.
METHODS
Neural stem cells were generated from the olfactory bulb of green fluorescent protein (GFP)-transgenic C57BL6 mice. Anosmia were induced by injection of intraperitoneal 3-methylindole. The neural stem cells were transplanted transnasally on the next day. The olfactory function was evaluated by a food-finding test once a week. The olfactory neuroepithelium was harvested for histologic examination and protein analysis at 4 weeks.
RESULTS
Twenty-five percent (6/24) of the control mice that were not transplanted with neural stem cells survived at 4 weeks while 67% (8/12) of the transplanted mice survived (P=0.029). The food finding test showed that the transplanted mice resumed finding food at 3 weeks while the control mice resumed finding food at 4 weeks. GFP-positive cells were observed in the olfactory neuroepithelium of the transplanted mice. Western blotting revealed that the olfactory marker protein expression was significantly lower in the control mice than that in the transplanted mice.
CONCLUSION
This study demonstrated that improvement of mouse survival was achieved and recovery of olfactory function was promoted by transnasal transplantation of neural stem cells in the anosmic mouse model. These results indicate that stem cells might be one of the future modalities for treating olfactory impairment.

Keyword

Neural stem cell; Anosmia; Transplantation; Smell

MeSH Terms

Animals
Blotting, Western
Mice
Neural Stem Cells
Olfaction Disorders
Olfactory Bulb
Olfactory Marker Protein
Skatole
Smell
Stem Cells
Transplants
Olfactory Marker Protein
Skatole

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