Lab Anim Res.  2010 Mar;26(1):121-125. 10.5625/lar.2010.26.1.121.

Doublecortin-immunoreactive Neuroblasts in Each Layer of the Main Olfactory Bulb After Transient Cerebral Ischemia in Gerbils

• Choi JH
• Yoo KY
• Park OK
• Lee CH
• Hwang IK
• Shin HC
• Won MH

• KMID: 1458950
• DOI: http://doi.org/10.5625/lar.2010.26.1.121

Abstract

Neurogenesis in the adult brain occurs continuously throughout life. The main olfactory bulb (MOB) is the first central relay of the olfactory system. We examined proliferation of newly generated cells in each layer of the gerbil MOB after 5 min of transient cerebral ischemia using doublecortin (DCX), a marker of neuronal progenitors. Many DCX immunoreactive neuroblasts were found in the all layers of the MOBs of control and ischemia groups. Ten to 15 days after ischemia/reperfusion, no difference in numbers of DCX immunoreactive neuroblasts was found in the MOB. Thirty days after ischemia/reperfusion, significant increase of DCX immunoreactive cells was observed in all layers of ischemic MOB. This result indicates that neuroblasts increase in the MOB from 30 days after transient cerebral ischemia in gerbils.

Keyword

Doublecortin; main olfactory bulb; migration; transient ischemia

MeSH Terms

Adult
Brain
Gerbillinae
Humans
Ischemia
Ischemic Attack, Transient
Neurogenesis
Neurons
Olfactory Bulb

Figure

• 

  Figure 1. Low magnification of DCX immunoreactivity in the gerbil MOB of control (A, C, E and G) and ischemia-operated groups (B, D, F and H) after ischemia/reperfusion (I/R). DCX immunoreactive cells are distributed throughout the MOB. EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; RMS, rostral migratory stream. Bar=200 µm.

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  Figure 2. High magnification of DCX immunoreactivity in the glomerular (GL), external plexiform layers (EPL), granule cell layers (GCL) and rostral migratory stream (RMS) in the gerbil MOB of control (A and B) and ischemia-operated groups (C–J). Arrows indicate DCX immunoreactive neuronal precursors. Note the increase in DCX immunoreactive neuroblasts in all layers of MOB 30 days after I/R. Bar=50 µm.

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  Figure 3. Changes in DCX immunoreactive neuroblasts in the GL (A), EPL (B) and GCL (C) after transient cerebral ischemia. (n=7 per group; ∗P<0.05, significantly different from the control-group). The bars indicate the means±SEM.

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